Atherosclerosis, characterized by the formation of fat-laden plaques, is a chronic inflammatory disease. ABCA1 promotes cholesterol efflux, reduces cellular cholesterol accumulation, and regulates anti-inflammatory activities in an apoA-I- or ANXA1-dependent manner. The latter activity occurs by mediating the efflux of ANXA1, which plays a critical role in anti-inflammatory effects, cholesterol transport, exosome and microparticle secretion, and apoptotic cell clearance.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 371.00 | |
500 μg | 5 days | $ 1,490.00 | |
1 mg | 5 days | $ 2,480.00 |
Description | Atherosclerosis, characterized by the formation of fat-laden plaques, is a chronic inflammatory disease. ABCA1 promotes cholesterol efflux, reduces cellular cholesterol accumulation, and regulates anti-inflammatory activities in an apoA-I- or ANXA1-dependent manner. The latter activity occurs by mediating the efflux of ANXA1, which plays a critical role in anti-inflammatory effects, cholesterol transport, exosome and microparticle secretion, and apoptotic cell clearance. |
Species | Mouse |
Expression System | E. coli |
Tag | C-His |
Accession Number | P10107 |
Synonyms | Calpactin-2, LPC1, Annexin-1, Annexin I, ANXA1, p35, Chromobindin-9, Calpactin II, ANX1, Annexin A1, Lipocortin I |
Construction | Recombinant Mouse ANXA1 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Met1-Asn346.[Accession |P10107] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 39.6 kDa same as Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Atherosclerosis, characterized by the formation of fat-laden plaques, is a chronic inflammatory disease. ABCA1 promotes cholesterol efflux, reduces cellular cholesterol accumulation, and regulates anti-inflammatory activities in an apoA-I- or ANXA1-dependent manner. The latter activity occurs by mediating the efflux of ANXA1, which plays a critical role in anti-inflammatory effects, cholesterol transport, exosome and microparticle secretion, and apoptotic cell clearance. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
ANXA1 Protein, Mouse, Recombinant (His) ANX 1 LPC 1 Calpactin-2 LPC1 Annexin-1 ANXA 1 Annexin I ANXA1 ANX-1 p35 Chromobindin-9 Calpactin II ANX1 LPC-1 Annexin A1 Lipocortin I ANXA-1 recombinant recombinant-proteins proteins protein