IL-12 Protein, Human, Recombinant (His)

Name: IL-12 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02109
Price: 87 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF) Copy Product Info

Synonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF

Catalog No. TMPY-02109 Copy Product Info

IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$87	-	In Stock
10 µg	$142	7-10 days	7-10 days
20 µg	$233	7-10 days	7-10 days
50 µg	$462	7-10 days	7-10 days
100 µg	$815	7-10 days	7-10 days
200 µg	$1,380	7-10 days	7-10 days
500 µg	$3,480	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL.
Description	IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P29459-1&NP_002178.2
Construction	A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Ile 23
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin	< 5 EU/mg of the protein.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF

Chemical Properties

Molecular Weight

60.13 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.