IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 462.00 | |
500 μg | 5 days | $ 3,000.00 |
Description | IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | P29459-1&NP_002178.2 |
Synonyms | IL12, NKSF1, IMD28, CLMF2, NKSF2, NKSF, CLMF, IMD29, IL-12B, IL12 p70, NFSK, P35, interleukin 12A, IL-12A |
Construction | The p35 subunit of Human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The p40 subunit of Human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Molecular Weight | 60.13 kDa (predicted) |
Endotoxin | < 5 EU/mg of the protein. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-12 Protein, Human, Recombinant (His) CLMF-2 IMD 28 IL12 NKSF1 IMD28 P 35 CLMF2 NKSF 2 NKSF-2 NKSF2 P-35 IMD-29 NKSF 1 IMD 29 IMD-28 NKSF CLMF IL 12 IMD29 IL-12B IL-12 IL12 p70 NFSK P35 interleukin 12A IL-12A CLMF 2 NKSF-1 recombinant recombinant-proteins proteins protein