IL-35 Protein, Human, Recombinant

Name: IL-35 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-03879
Rating: 4.5 (1 reviews)

(Synonyms: P35, NKSF1, NFSK, Interleukin-35, interleukin 12A, IL-35, IL-12A, CLMF) Copy Product Info

Synonyms: P35, NKSF1, NFSK, Interleukin-35, interleukin 12A, IL-35, IL-12A, CLMF

Catalog No. TMPY-03879 Copy Product Info

IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1.

Pack Size	Price	USA Stock	Global Stock
5 μg	$112	7-10 days	7-10 days
10 μg	$188	7-10 days	7-10 days
20 μg	$297	7-10 days	7-10 days
50 μg	$618	7-10 days	7-10 days
100 μg	$939	7-10 days	7-10 days
200 μg	$1,430	7-10 days	7-10 days
500 μg	$2,880	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P29459-1
Construction	A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a His tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Arg 21
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	P35, NKSF1, NFSK, Interleukin-35, interleukin 12A, IL-35, IL-12A, CLMF
Research Background

Chemical Properties

Molecular Weight

102 kDa (predicted); 60 & 63 kDa (reducing conditions)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

P-35P 35NKSF-1NKSF 1IL35IL 35

Related Tags: IL-35 Protein, Human, Recombinant chemical structure | IL-35 Protein, Human, Recombinant molecular weight