CD32A Protein, Human, Recombinant (R167, His)

Catalog No. TMPJ-00446

Synonyms: FcRII-a, IGFR2, Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, Fc-Gamma RII-a, FCGR2A1, Fc-γ RII-a, FCGR2A, CDw32, FCG2, Fc-Gamma-RIIa, CD32, Low Affinity Immunoglobulin γ Fc Region Receptor II-a, IgG Fc receptor II-a, Fc-γ-RIIa

Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating type receptor either has or associates noncovalently with an accessory subunit that has an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

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CD32A Protein, Human, Recombinant (R167, His)

Pack Size	Availability	Price/USD
10 μg	5 days	$ 97.00
50 μg	5 days	$ 272.00
500 μg	5 days	$ 1,900.00
1 mg	5 days	$ 2,730.00

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DATASHEET SDS

Biological Description

Technical Params

Product Properties

Description

Species	Human
Expression System	Human Cells
Tag	C-6His
Accession Number	AAA35827
Synonyms	FcRII-a, IGFR2, Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, Fc-Gamma RII-a, FCGR2A1, Fc-γ RII-a, FCGR2A, CDw32, FCG2, Fc-Gamma-RIIa, CD32, Low Affinity Immunoglobulin γ Fc Region Receptor II-a, IgG Fc receptor II-a, Fc-γ-RIIa
Amino Acid	Ala36-Ile218
Construction	Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-A(R167) is produced by our Mammalian expression system and the target gene encoding Ala36-Ile218 is expressed with a 6His tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Molecular Weight	25-32 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating type receptor either has or associates noncovalently with an accessory subunit that has an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

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Keywords

CD32A Protein, Human, Recombinant (R167, His) FcRII-a IGFR2 Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a Fc-Gamma RII-a FCGR2A1 Fc-γ RII-a IGFR 2 FCGR2A CDw32 FCG2 Fc-Gamma-RIIa CD32 Low Affinity Immunoglobulin γ Fc Region Receptor II-a CD 32 IgG Fc receptor II-a IGFR-2 Fc-γ-RIIa FCG 2 FCG-2 CD-32 recombinant recombinant-proteins proteins protein