CD32B/Fcgr2b Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00800

CD32B/Fcgr2b Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.7 kDa and the accession number is A0A0B4J1E6.

CD32B/Fcgr2b Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00800

CD32B/Fcgr2b Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.7 kDa and the accession number is A0A0B4J1E6.

Pack Size	Price	Availability
100 μg	$386	In Stock
200 μg	$682	7-10 days
500 μg	$1,440	7-10 days

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Product Information

Biological Activity	1.Loaded Mouse FcγRIIB / CD32b recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.1 μM as determined in a BLI assay. 2. The purity of Mouse CD32 / FCGR2B Protein (His Tag) is more than 95% and the molecular weight of this protein is around 26-38 kDa verified by SEC-MALS. 3.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse CD32B/Fcgr2b Protein, His Tag with an affinity constant of 1.07µM as determined in BLI assay(QC tested).
Description	CD32B/Fcgr2b Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.7 kDa and the accession number is A0A0B4J1E6.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A0A0B4J1E6
Synonyms	Ly-m20,LyM-1,Ly-17,fcRII,Fcr-3,Fcr-2,FcgRII,Fcgr2a,Fcgr2,Fc[g]RII,Fc fragment of IgG, low affinity IIb, receptor (CD32),F630109E10Rik,CD32,AI528646
Construction	A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FCGR2B (NP_001070657.1) precursor was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Thr 40
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Molecular Weight	21.7 kDa (predicted); 34 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.