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CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated

TargetMol | SPR
Catalog No. TMPY-01986 Copy Product Info
CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.9 kDa and the accession number is A0A0B4J1E6.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$1677-10 days7-10 days
10 μg$2777-10 days7-10 days
20 μg$4627-10 days7-10 days
50 μg$7467-10 days7-10 days
100 μg$1,2307-10 days7-10 days
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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Information

Biological Activity
Loaded Recombinant Mouse CD32B/Fcgr2b Protein, His Tag, Biotinylated (Cat#TMPY-01986) on SA Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 2.32µM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
Description
CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.9 kDa and the accession number is A0A0B4J1E6.
Species
Mouse
Expression System
HEK293 Cells
TagHis, Avi
Accession NumberA0A0B4J1E6
Synonyms
Ly-m20,LyM-1,Ly-17,fcRII,Fcr-3,Fcr-2,FcgRII,Fcgr2a,Fcgr2,Fc[g]RII,Fc fragment of IgG, low affinity IIb, receptor (CD32),F630109E10Rik,CD32,AI528646
Construction
A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FGGR2B (NP_001070657.1) as fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Thr 40
Protein Purity
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Molecular Weight23.9 kDa (predicted); 38-43 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

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