Shopping Cart
Remove All
  • TargetMol
    Your shopping cart is currently empty

CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated

(Synonyms: Ly-m20, LyM-1, Ly-17, fcRII, Fcr-3, Fcr-2, FcgRII, Fcgr2a, Fcgr2, Fc[g]RII, Fc fragment of IgG, low affinity IIb, receptor (CD32), F630109E10Rik, CD32, AI528646) Copy Product Info

Synonyms: Ly-m20, LyM-1, Ly-17, fcRII, Fcr-3, Fcr-2, FcgRII, Fcgr2a, Fcgr2, Fc[g]RII, Fc fragment of IgG, low affinity IIb, receptor (CD32), F630109E10Rik, CD32, AI528646

Catalog No. TMPY-01986 Copy Product Info
CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.9 kDa and the accession number is A0A0B4J1E6.

Product Introduction

Bioactivity
Bioactivity
Loaded Recombinant Mouse CD32B/Fcgr2b Protein, His Tag, Biotinylated (Cat#TMPY-01986) on SA Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 2.32µM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
Description
CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.9 kDa and the accession number is A0A0B4J1E6.
Species
Mouse
Expression System
HEK293 Cells
TagHis, Avi
Accession NumberA0A0B4J1E6
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FGGR2B (NP_001070657.1) as fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Thr 40
Protein Purity
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsLy-m20, LyM-1, Ly-17, fcRII, Fcr-3, Fcr-2, FcgRII, Fcgr2a, Fcgr2, Fc[g]RII, Fc fragment of IgG, low affinity IIb, receptor (CD32), F630109E10Rik, CD32, AI528646
Chemical Properties
Molecular Weight23.9 kDa (predicted); 38-43 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Keywords

Related Tags: CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated chemical structure | CD32B/Fcgr2b Protein, Mouse, Recombinant (His & Avi), Biotinylated molecular weight