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CD32A Protein, Human, Recombinant (167 His, His)

(Synonyms: IGFR2, FCGR2A1, FCGR2, FcGR, FCG2, Fc γ RIIA, Fc gamma RIIA, Fc fragment of IgG receptor IIa, CDw32, CD32A, CD32) Copy Product Info

Synonyms: IGFR2, FCGR2A1, FCGR2, FcGR, FCG2, Fc γ RIIA, Fc gamma RIIA, Fc fragment of IgG receptor IIa, CDw32, CD32A, CD32

Catalog No. TMPY-01968 Copy Product Info
CD32A Protein, Human, Recombinant (167 His, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22 kDa and the accession number is P12318-1.

Batch Information

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Purity:SDS-PAGE: 98.3%; SEC-HPLC: 96.8%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
1.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRIIA / CD32a (H167) recombinant protein with an affinity constant of 1.7 μM as determined in a BLI assay. 2.Loaded FcγRIIA / CD32a (H167) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.55 μM as determined in a BLI assay. 3.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIA / CD32a (H167) recombinant protein with an affinity constant of 0.58 µM as determined in an SPR assay.
Description
CD32A Protein, Human, Recombinant (167 His, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22 kDa and the accession number is P12318-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP12318-1
ConstructionA DNA sequence encoding the extracellular domain (Met1-Ile218) of human FCGR2A (P12318-1) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gln 34
Protein Purity
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
CD32A Protein, Human, Recombinant (167 His, His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsIGFR2, FCGR2A1, FCGR2, FcGR, FCG2, Fc γ RIIA, Fc gamma RIIA, Fc fragment of IgG receptor IIa, CDw32, CD32A, CD32
Chemical Properties
Molecular Weight22 kDa (predicted); 30 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD32A Protein, Human, Recombinant (167 His, His) chemical structure | CD32A Protein, Human, Recombinant (167 His, His) molecular weight