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Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated

(Synonyms: IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32) Copy Product Info

Synonyms: IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32

Catalog No. TMPK-00200 Copy Product Info
Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.

Product Introduction

Bioactivity
Bioactivity
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB, His-Avi Tag with an affinity constant of 3.97 μM as determined in SPR assay.
Description
Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP31994-1
ConstructionAla46-Pro217
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsIGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32
Research Background
Chemical Properties
Molecular Weight22.5 kDa (predicted). Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight