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Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated
(Synonyms: IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32) Copy Product InfoSynonyms: IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32
Catalog No. TMPK-00200 Copy Product Info
Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $91 | 7-10 days | 7-10 days | |
| 10 µg | $148 | 7-10 days | 7-10 days | |
| 20 µg | $245 | 7-10 days | 7-10 days | |
| 50 µg | $483 | 7-10 days | 7-10 days | |
| 100 µg | $813 | 7-10 days | 7-10 days | |
| 200 µg | $1,470 | 7-10 days | 7-10 days | |
| 500 µg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB, His-Avi Tag with an affinity constant of 3.97 μM as determined in SPR assay. |
| Description | Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P31994-1 |
| Construction | Ala46-Pro217 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32 |
| Research Background | The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens. |
| Molecular Weight | 22.5 kDa (predicted). Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight
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