Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 814.00 | |
500 μg | 5 days | $ 3,250.00 | |
1 mg | 5 days | $ 5,440.00 |
Description | Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P31994-1 |
Synonyms | Fc γ RII, FCG2, FCGR2, Fc gamma RIIB, CD32, Fc gamma RII, FCGR2A, Fc γ RIIB, FCGR21, CD32b, IGFR2, CDw32, CD32A, FcGR |
Construction | Recombinant Biotinylated Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala46-Pro217.[Accession |P31994-1] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens. |
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Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi), Biotinylated Fc γ RII FCGR-21 FCG2 FCGR2 FCGR-2 FCG-2 Fc gamma RIIB FCG 2 CD32 Fc gamma RII FCGR2A FCGR 21 Fc γ RIIB IGFR-2 FCGR 2 FCGR21 CD 32 CD32b IGFR2 CD-32 CDw32 CD32A FcGR IGFR 2 recombinant recombinant-proteins proteins protein