Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi)

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Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00209

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 23.2 kDa and the accession number is P12318-1.

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Pack Size	Price	Availability
100 μg	$371	7-10 days
500 μg	$1,490	7-10 days
1 mg	$2,480	7-10 days

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Biological Description

Biological Information	Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His Tag with an affinity constant of 0.73 μM as determined in SPR assay.
Description	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 23.2 kDa and the accession number is P12318-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P12318-1
Synonyms	FCGR2,FCRIIA,Fc γ RIIA,FCGR2A1,FCGR2A,FcgRIIA,Fc gamma RIIA,CD32A,FCG2,fcRII-a
Construction	Ala36-Ile218(H167)
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	23.2 kDa (predicted). Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

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Keywords

FCGR 2 FCG-2 FCGR-2 FCG 2