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CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi)

TargetMol | SPR
Catalog No. TMPK-00199 Copy Product Info
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.

CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00199
Copy Product Info
TargetMol | SPR

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.

CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi)
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$46-In Stock
10 μg$72-In Stock
20 μg$115-In Stock
50 μg$2227-10 days7-10 days
100 μg$371-In Stock
200 μg$6737-10 days7-10 days
500 μg$1,4907-10 days7-10 days
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In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Information

Biological Activity
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB, His Tag with an affinity constant of 3.46 μM as determined in SPR assay.
Description
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP31994-1
Synonyms
IGFR2,FCGR2A,FCGR21,FCGR2,FcGR,FCG2,Fc γ RIIB,Fc γ RII,Fc gamma RIIB,Fc gamma RII,CDw32,CD32b,CD32A,CD32
Construction
Ala46-Pro217
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight22.5 kDa (predicted). Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

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