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CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi)

(Synonyms: IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32) Copy Product Info

Synonyms: IGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32

Catalog No. TMPK-00199 Copy Product Info
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.

Batch Information

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Purity:> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB, His Tag with an affinity constant of 3.46 μM as determined in SPR assay.
Description
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RIIB/CD32b Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.5 kDa and the accession number is P31994-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP31994-1
ConstructionAla46-Pro217
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsIGFR2, FCGR2A, FCGR21, FCGR2, FcGR, FCG2, Fc γ RIIB, Fc γ RII, Fc gamma RIIB, Fc gamma RII, CDw32, CD32b, CD32A, CD32
Research Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Chemical Properties
Molecular Weight22.5 kDa (predicted). Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi) chemical structure | CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi) molecular weight