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Tubulin polymerization-IN-13

Catalog No. T61482 CAS 2426665-56-5

Tubulin polymerization-IN-13 (Compound 4f) is a potent inhibitor of tubulin polymerization, with an IC50 value of 0.37 μM. It displays significant anti-proliferative effects against cancer cells, inducing apoptosis and exhibiting potential antivascular activity [1].

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Tubulin polymerization-IN-13 Chemical Structure

Tubulin polymerization-IN-13, CAS 2426665-56-5

Pack Size	Availability	Price/USD
25 mg	6-8 weeks	$ 1,520.00
50 mg	6-8 weeks	$ 1,980.00
100 mg	6-8 weeks	$ 2,500.00

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Description	Tubulin polymerization-IN-13 (Compound 4f) is a potent inhibitor of tubulin polymerization, with an IC50 value of 0.37 μM. It displays significant anti-proliferative effects against cancer cells, inducing apoptosis and exhibiting potential antivascular activity [1].
In vitro	Tubulin Polymerization-IN-13, at concentrations ranging from 0.005 to 2.8 nM, effectively inhibits the proliferation of tumor cells, while exhibiting non-toxic effects on non-tumor cells at concentrations between 8.7 and 10 μM. Furthermore, treatment with Tubulin Polymerization-IN-13 at doses of 1 to 50 nM for 24 hours leads to cell cycle arrest at the G2/M phase, and a concentration of 10 nM induces apoptosis within 24 and 48 hours of treatment. This compound also causes alterations in the microtubule network at doses between 10 and 100 nM after 24 hours of exposure. In depth analysis reveals IC50 values of 2.8 nM, 2.1 nM, 0.005 nM, 2.7 nM, 0.31 nM, and 0.28 nM for the cell lines HeLa, HT-29, Daoy, HL-60, SEM, and Jurkat, respectively, indicating its efficacy in halting cell proliferation. Additionally, the compound has shown a GI50 of 8.7 μM in quiescent lymphocytes, demonstrating its safety in non-cancerous cells. Detailed cell cycle, apoptosis, and immunofluorescence analyses on HeLa cells underline Tubulin Polymerization-IN-13's mechanism of action, which includes G2/M arrest, activation of caspase-9, cleavage of PARP, phosphorylation of Bcl-2, downregulation of Mcl-1, and disorganization of microtubules, further establishing its potential therapeutic benefits.
In vivo	Tubulin polymerization-IN-13, administered through intraperitoneal injection at doses of 15 or 5 mg/kg once every other day for a total of four administrations, significantly diminished tumor growth in a dose-dependent manner within an orthotopic murine model of mammary carcinoma (E0771 cell line) implanted in seven-week-old female C57BL/6 mice. The treatment resulted in a 45.7% reduction in tumor mass at 15 mg/kg and a 16.9% reduction at 5 mg/kg, without any observed toxicity or decrease in body weight of the animals [1].

Molecular Weight	371.38
Formula	C20H21NO6
CAS No.	2426665-56-5

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Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Tubulin polymerization-IN-13 2426665-56-5 inhibitor inhibit

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