Tocilizumab

Tocilizumab (Anti-Human IL6R) is a neutralizing antibody against human interleukin-6 receptor (IL-6R) that blocks the binding of IL-6 to IL-6R, thereby inhibiting both classical and trans signaling. It can be used to study rheumatoid arthritis and has been suggested as a possible drug for the treatment of severe COVID-19.

METHODS: Tocilizumab (Anti-Human IL6R) (0.001–10,000 μg/ml) was used to culture Ba/F3-gp130-hIL-6R or Ba/F3-gp130-mIL-6R cells for 2 days, and cell proliferation was observed.
RESULTS Tocilizumab (Anti-Human IL6R) could inhibit cell proliferation, with an IC50 value of 13.5 ng/mL. [1]

METHODS: HARA-B cells were inoculated into the left ventricle of nude mouse hearts. Three weeks after inoculation, Tocilizumab (Anti-Human IL6R) was intravenously injected weekly for the next three weeks (1.0 mg/100 μL, once every two weeks for three weeks).
RESULTS The volume of each metastatic lesion in the brains of mice injected with Tocilizumab (Anti-Human IL6R) was significantly smaller. [2]

Objective: To validate that IL-6 signaling is essential for TAM (tumor-associated macrophages)-enhanced CSC (cancer stem cells) function. Concentrations: 5 μg/ml. Incubation Time: 72 h. Method: For co-culture experiments, 12-well Transwell chambers were used with 2×105 HepG2 or Hep3B cells seeded in the lower chamber one day before co-culture and either 5×105 or 1×106 TAMs added in the upper chamber. Tocilizumab or human IgG antibody were added in indicated experiments to selected wells. After 24 to 72 hours, HCC cells and CD14+ TAM cells were collected separately for analysis. Cells:HepG2 cell lines, TAMs isolated from HCCs (hepatocellular carcinoma)

Human

Immobilized Human IL-6R alpha/CD126 Protein (His) (TMPY-00864) at 1 μg/mL (100 μL/well) can bind Tocilizumab. The EC50 is 2.610 ng/mL.

Monoclonal

Unconjugated