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Sialyl-Lewis X

Catalog No. T35884 CAS 98603-84-0

Sialyl-Lewis X (sLeX), a sialylated fucosylated tetrasaccharide and endogenous antigen, serves as a high-affinity ligand for selectins (E-, P-, and L-selectin)[1]. It interacts with ELAM-1 and CD62, consequently inhibiting CD62-mediated neutrophil recruitment to inflammation sites[2].

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Sialyl-Lewis X, CAS 98603-84-0

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Description

In vitro

Sialyl-Lewis X (sLeX) serves as a high-affinity ligand for CD62. The use of antibodies, specifically mAb CSLEX (IgM; anti-sLeX), obstructs the CD62-mediated attachment of HL-60 cells to activated platelets. Moreover, liposomes integrated with glycolipids mimicking the sLeX configuration are effective in preventing the adhesion of HL-60 cells and human neutrophils. Notably, sLeX liposomes demonstrate a superior inhibitory capability on cell adhesion, exhibiting a tenfold greater affinity compared to Lex liposomes, achieving maximum inhibition at a lower concentration (1 µg/ml) compared to a partial (50%) inhibition achieved by Lex liposomes at a higher concentration (5 µg/ml). Additionally, a soluble human milk oligosaccharide containing the LeX structure also impedes the binding of neutrophils to activated platelets, wherein sLeX is identified as a substantially more effective inhibitor (30-fold) than its nonsialylated counterpart, Lex. Specifically, to attain a 50% reduction in neutrophil adhesion, sLeX requires only 2 µg/ml, in contrast to Lex, which necessitates 54 µg/ml.

Molecular Weight	820.748
Formula	C31H52N2O23
CAS No.	98603-84-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. M J Polley, et al. CD62 and Endothelial Cell-Leukocyte Adhesion Molecule 1 (ELAM-1) Recognize the Same Carbohydrate Ligand, sialyl-Lewis X. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6224-8. 2. L A Lasky, et al. Selectins: Interpreters of Cell-Specific Carbohydrate Information During Inflammation. Science

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Keywords

Sialyl-Lewis X 98603-84-0 SialylLewis X Sialyl Lewis X inhibitor inhibit

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