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S130

Name: S130
Brand: TargetMol
SKU: T12819
Rating: 4.5 (1 reviews)

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Catalog No. T12819Cas No. 1160852-22-1

S130 is a high affinity, selectiveATG4B (a major cysteine protease) inhibitor (IC50 of 3.24 μM).It suppresses autophagy flux.

S130

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Catalog No. T12819Cas No. 1160852-22-1

S130 is a high affinity, selectiveATG4B (a major cysteine protease) inhibitor (IC50 of 3.24 μM).It suppresses autophagy flux.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$297	6-8 weeks	6-8 weeks
25 mg	$987	6-8 weeks	6-8 weeks
50 mg	$1,360	6-8 weeks	6-8 weeks
100 mg	$1,860	6-8 weeks	6-8 weeks
1 mL x 10 mM (in DMSO)	$318	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	S130 is a high affinity, selectiveATG4B (a major cysteine protease) inhibitor (IC50 of 3.24 μM).It suppresses autophagy flux.
Targets&IC50	ATG4B:3.24 µM
In vitro	S130 suppresses autophagy and activates apoptosis by inhibiting ATG4B, leading to enhanced cytotoxicity. S130 (0-25 μM; 48 hours) induces cell death through ATG4B inhibition at doses higher than 6.3 μM, without causing necroptosis. Nutrient deprivation enhances S130-induced cytotoxicity. S130 (0-10 μM; 24 hours) suppresses approximately 79% of full-length LC3-GST cleavage at 10 μM, while no substrates were processed in ATG4B KO cells. S130 exhibits significant inhibitory effects on ATG4B.
In vivo	Tumor growth suppressed by S130 (20 mg/kg; i.p.; daily; 3 weeks), S130 shows an efficient in vivo antitumor effect with a sound safety on vital organs.

Chemical Properties

Molecular Weight	387.47
Formula	C₂₄H₂₅N₃O₂
Cas No.	1160852-22-1
Smiles	CCN(CC)CCCNC(=O)c1cnc2C(=O)c3ccccc3-c3cccc1c23
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (322.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (8.52 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5808 mL	12.9042 mL	25.8084 mL	129.0422 mL
5 mM	0.5162 mL	2.5808 mL	5.1617 mL	25.8084 mL
10 mM	0.2581 mL	1.2904 mL	2.5808 mL	12.9042 mL
20 mM	0.1290 mL	0.6452 mL	1.2904 mL	6.4521 mL
50 mM	0.0516 mL	0.2581 mL	0.5162 mL	2.5808 mL
100 mM	0.0258 mL	0.1290 mL	0.2581 mL	1.2904 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc