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Phenolphthalein

Name: Phenolphthalein
Brand: TargetMol
SKU: T0017
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0017Cas No. 77-09-8

Alias Phthalin, Phthalimetten, Euchessina

Phenolphthalein (Phthalin) is an acid-base indicator which is colorless in acid solution, but turns pink to red as the solution becomes alkaline. It is used medicinally as a cathartic.

Phenolphthalein

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Purity: 99.89%

Catalog No. T0017Alias Phthalin, Phthalimetten, EuchessinaCas No. 77-09-8

Phenolphthalein (Phthalin) is an acid-base indicator which is colorless in acid solution, but turns pink to red as the solution becomes alkaline. It is used medicinally as a cathartic.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 g	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$42	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.89%

Appearance:Solid

Color:White

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COA HNMR HPLC

Product Introduction

Phenolphthalein AI Summary

Phenolphthalein exhibits a diverse range of bioactivities across various biological targets and pathways. It acts as an inhibitor of multiple thymidylate synthase enzymes, including those from Lactobacillus casei (Ki = 4700.0 nM, IF = 0.444), Pneumocystis carinii (IF = 0.608), and humans (IF = 0.182). The compound also shows cytotoxicity against mock-infected MT-4 cells (CC50 = 58000.0 nM) and inhibits the growth of several pathogens such as Staphylococcus aureus (MIC > 58000.0 nM), Streptococcus (MIC = 38000.0 nM; MGC = 58.0 uM), and Cryptococcus neoformans (MIC > 58000.0 nM). Additionally, it has shown effects on the accumulation of radioactivity in COS-7 cells and displays minimal differences in logD between pH 6.5 and 7.4, indicating a partition coefficient of 2.41. In terms of enzyme inhibition, Phenolphthalein demonstrates activity against UGT enzymes, notably UGT1A8 and UGT1A9, and has moderate action against OATP1B1 and OATP1B3 at 10 uM. It also has some effectiveness against SARS-CoV-2, inhibiting virus-induced cytotoxicity in Vero and Caco-2 cells, though with a relatively high IC50 (greater than 20,000 nM). Furthermore, it inhibits the SARS-CoV-2 3CL-Pro protease at 20µM. Despite its multiple activities, the compound shows limited oral bioavailability in humans (F ≤ 20%) and low effectiveness in inhibiting certain targets, including the HDAC6 enzyme and SARS-CoV-2 in specific cell lines. The bioactivity spectrum of Phenolphthalein indicates its potential utility as a multifaceted inhibitor with applications in antibacterial, antiviral, and enzyme inhibition therapies, though its clinical utility may be constrained by its moderate potency and bioavailability..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Phenolphthalein (Phthalin) is an acid-base indicator which is colorless in acid solution, but turns pink to red as the solution becomes alkaline. It is used medicinally as a cathartic.
In vitro	Instructions Phenolphthalein indicator (0.5% phenolphthalein ethanol solution) preparation method: Take 0.5g phenolphthalein, dissolve it in 95% ethanol, no need to add water, dilute to 100mL. Color change range: pH8.3～10.0 (colorless→red), add 1～2 drops to 100mL titration solution. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Phthalin, Phthalimetten, Euchessina

Chemical Properties

Molecular Weight	318.32
Formula	C₂₀H₁₄O₄
Cas No.	77-09-8
Smiles	OC1=CC=C(C=C1)C1(OC(=O)C2=C1C=CC=C2)C1=CC=C(O)C=C1
Relative Density.	Ca. 1.296. Temperature:20.6 °C.

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (188.49 mM), Sonication and heating are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (7.85 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1415 mL	15.7075 mL	31.4149 mL	157.0746 mL
5 mM	0.6283 mL	3.1415 mL	6.2830 mL	31.4149 mL
10 mM	0.3141 mL	1.5707 mL	3.1415 mL	15.7075 mL
20 mM	0.1571 mL	0.7854 mL	1.5707 mL	7.8537 mL
50 mM	0.0628 mL	0.3141 mL	0.6283 mL	3.1415 mL
100 mM	0.0314 mL	0.1571 mL	0.3141 mL	1.5707 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc