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PF-06465469

Name: PF-06465469
Brand: TargetMol
SKU: T16493
Price: 45 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16493Cas No. 1407966-77-1

Alias PF06465469, PF 06465469

PF-06465469 is a covalent ITK inhibitor (IC₅₀ = 2 nM) that also inhibits BTK. PF-06465469 suppresses CXCL12-mediated cell migration, decreases PD-1 and LAG-3 expression. PF-06465469 shows strong potential application in leukemia and T-cell lymphoma research.

PF-06465469

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Catalog No. T16493Alias PF06465469, PF 06465469Cas No. 1407966-77-1

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$45	-	In Stock
5 mg	$106	-	In Stock
10 mg	$179	-	In Stock
25 mg	$392	-	In Stock
1 mL x 10 mM (in DMSO)	$123	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	PF-06465469 is a covalent ITK inhibitor (IC₅₀ = 2 nM) that also inhibits BTK. PF-06465469 suppresses CXCL12-mediated cell migration, decreases PD-1 and LAG-3 expression. PF-06465469 shows strong potential application in leukemia and T-cell lymphoma research.
Targets&IC50	ITK:2 nM
In vitro	Treatment of Jurkat cells with 2 µM PF-06465469 for 24 hours reduced intracellular ITK phosphorylation levels and inhibited the migratory response of Jurkat cells to CXCL12 [1]. When the concentration of PF-06465469 was 1 µM, it significantly downregulated the expression levels of CD69, PD-1, and LAG-3 molecules in CAR-T cells co-cultured with MEC1 cells [2].
Synonyms	PF06465469, PF 06465469

Chemical Properties

Molecular Weight	523.63
Formula	C₃₀H₃₃N₇O₂
Cas No.	1407966-77-1
Smiles	NC1=C2C(=NN(C2=NC=N1)[C@H]3CN(C(C=C)=O)CCC3)C4=CC(C(NC5=CC(C)=C(C(C)C)C=C5)=O)=CC=C4
Relative Density.	1.30 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 8.5 mg/mL (16.23 mM), Sonication is recommeded.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9097 mL	9.5487 mL	19.0975 mL	95.4873 mL
5 mM	0.3819 mL	1.9097 mL	3.8195 mL	19.0975 mL
10 mM	0.1910 mL	0.9549 mL	1.9097 mL	9.5487 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc