Shopping Cart

Your shopping cart is currently empty

PF-05180999

Name: PF-05180999
Brand: TargetMol
SKU: T16484
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T16484Cas No. 1394033-54-5

Alias PF-999, PF999, PF-5180999, PF5180999, PF05180999

PF-05180999 (PF-999) is a selective and potent phosphodiesterase 2A (PDE2A) inhibitor that enhances long-term memory in a contextual fear conditioned reflex model in rats.

PF-05180999

🥰Excellent

Purity: 99.82%

Catalog No. T16484Alias PF-999, PF999, PF-5180999, PF5180999, PF05180999Cas No. 1394033-54-5

PF-05180999 (PF-999) is a selective and potent phosphodiesterase 2A (PDE2A) inhibitor that enhances long-term memory in a contextual fear conditioned reflex model in rats.

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$40	-	In Stock
5 mg	$64	-	In Stock
10 mg	$113	-	In Stock
25 mg	$239	-	In Stock
50 mg	$396	-	In Stock
100 mg	$691	-	In Stock
1 mL x 10 mM (in DMSO)	$71	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.82%

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	PF-05180999 (PF-999) is a selective and potent phosphodiesterase 2A (PDE2A) inhibitor that enhances long-term memory in a contextual fear conditioned reflex model in rats.
Targets&IC50	PDE11A4:50.09 μM, PDE2A (dog):8.4 nM (Ki), PDE10A1:2.03 μM, PDE2A (monkey):3.4 nM, PDE7B:26.969 μM, PDE2A (dog):5.2 nM, PDE2A (monkey):5.5 nM (Ki), PDE2A (rat):2.6 nM, PDE2A (rat):4.2 nM (Ki)
In vitro	PF-05180999 is a weak inducer of CYP3A4 with no direct inhibition of human recombinant cytochrome P450 enzymes (including 1A2, 2B6, 2C8, 2C9, 2C19, 2D6, and 3A) and does not induce CYP1A2.PF-05180999 binds to PDE2A in rat, dog, and monkey with Ki values of 4.2 nM 8.4 nM, and 5.5 nM, with IC50 values of 2.6 nM, 5.2 nM, and 3.4 nM, respectively.For the other PDE isoforms, PF-05180999 was weakly active, with IC50s of 2.03 μM (PDE10A1), 26.969 μM (PDE7B), 50.09 μM (PDE11A4), and >56.25 μM (PDE1B1, PDE3A1, PDE4D3, PDE5A1, PDE6 (bovine), PDE8B, PDE9A1). [1]
In vivo	PF-05180999 induced an acute, dose-dependent increase in cGMP levels in mouse cortex, striatum and hippocampus, but had no effect on cAMP levels and its downstream phosphorylated cAMP response element binding protein (p-CREB). In the radial arm maze (RAM) model of working memory, subcutaneous injection of 0.032-0.32 mg/kg PF-05180999 significantly reduced ketamine-induced working memory errors in rats. [1]
Synonyms	PF-999, PF999, PF-5180999, PF5180999, PF05180999

Chemical Properties

Molecular Weight	414.39
Formula	C₁₉H₁₇F₃N₈
Cas No.	1394033-54-5
Smiles	FC(F)(F)C=1C=NC(=CC1)C2=C(C=NN2C)C=3N=C(N4N=CN=C(C34)N5CCC5)C
Relative Density.	1.57 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (96.53 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.83 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4132 mL	12.0659 mL	24.1319 mL	120.6593 mL
5 mM	0.4826 mL	2.4132 mL	4.8264 mL	24.1319 mL
10 mM	0.2413 mL	1.2066 mL	2.4132 mL	12.0659 mL
20 mM	0.1207 mL	0.6033 mL	1.2066 mL	6.0330 mL
50 mM	0.0483 mL	0.2413 mL	0.4826 mL	2.4132 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc