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para-Nitroblebbistatin

Name: para-Nitroblebbistatin
Brand: TargetMol
SKU: T12360
Price: 89 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12360Cas No. 1621326-32-6

Alias p-Nitroblebbistatin

para-Nitroblebbistatin is a non-cytotoxic, non-fluorescent, photostable, and specific inhibitor of myosin II. para-Nitroblebbistatin can be used to study the specific roles of myosin II in physiology, development, and cell biology.

para-Nitroblebbistatin

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Purity: 99.84%

Catalog No. T12360Alias p-NitroblebbistatinCas No. 1621326-32-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$89	In Stock	In Stock
5 mg	$223	In Stock	In Stock
10 mg	$333	In Stock	In Stock
25 mg	$538	In Stock	In Stock
50 mg	$732	-	In Stock
100 mg	$990	-	In Stock
200 mg	$1,330	-	In Stock
1 mL x 10 mM (in DMSO)	$227	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.84%

Appearance:Solid

Color:Orange

Resource Download

COA HNMR LCMS

Product Introduction

para-Nitroblebbistatin AI Summary

para-Nitroblebbistatin exhibits moderate apparent permeability in human Caco2 cells, with Papp values of 32.5 x 10^-6 cm/s from the apical to basolateral side and 2.23 x 10^-6 cm/s from the basolateral to apical side. It demonstrates good drug recovery, achieving 34.0% recovery from the apical to basolateral side and 72.0% recovery from the basolateral to apical side in Caco2 cells. The compound shows relatively high aqueous solubility in pH 7.4 PBS, with a concentration of 31700.0 nM after 24 hours. Additionally, para-Nitroblebbistatin is photostable, with a half-life greater than 1.5 hours under irradiation at 390 to 470 nm in a DMSO/H2O solvent. Regarding bioactivity, para-Nitroblebbistatin inhibits rabbit skeletal muscle myosin 2 with an IC50 of 400.0 nM, and Dictyostelium discoideum myosin 2 ATPase activity with an IC50 of 2300.0 nM in NADH-coupled assays. It also acts as an inhibitor of actin-activated rabbit skeletal muscle myosin 2 with an IC50 of 500.0 nM and of actin-activated bovine heart muscle myosin 2 with an IC50 of 2100.0 nM. The selectivity ratio of IC50 for bovine heart muscle myosin 2 to rabbit skeletal muscle myosin 2 is 4.2, indicating some level of target selectivity..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	para-Nitroblebbistatin is a non-cytotoxic, non-fluorescent, photostable, and specific inhibitor of myosin II. para-Nitroblebbistatin can be used to study the specific roles of myosin II in physiology, development, and cell biology.
In vitro	METHODS: HeLa cells were treated with para-Nitroblebbistatin (p-Nitroblebbistatin) (20 μM, 3 days) and the changes in cell number and death rate were tracked. RESULTS para-Nitroblebbistatin effectively inhibited cytokinesis and had no effect on cell number and death rate. [1]
Synonyms	p-Nitroblebbistatin

Chemical Properties

Molecular Weight	337.33
Formula	C₁₈H₁₅N₃O₄
Cas No.	1621326-32-6
Smiles	Cc1ccc2N=C3N(CC[C@@]3(O)C(=O)c2c1)c1ccc(cc1)[N+]([O-])=O
Relative Density.	1.47 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 25 mg/mL (74.11 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9645 mL	14.8223 mL	29.6446 mL	148.2228 mL
5 mM	0.5929 mL	2.9645 mL	5.9289 mL	29.6446 mL
10 mM	0.2964 mL	1.4822 mL	2.9645 mL	14.8223 mL
20 mM	0.1482 mL	0.7411 mL	1.4822 mL	7.4111 mL
50 mM	0.0593 mL	0.2964 mL	0.5929 mL	2.9645 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc