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OT-82

Name: OT-82
Brand: TargetMol
SKU: T12330
Price: 147 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12330Cas No. 1800487-55-1

Alias OT82, OT 82

OT-82 is a potent, selective, and orally active inhibitor of nicotinamide phosphoribosyltransferase (NAMPT).

OT-82

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Purity: 99.31%

Catalog No. T12330Alias OT82, OT 82Cas No. 1800487-55-1

OT-82 is a potent, selective, and orally active inhibitor of nicotinamide phosphoribosyltransferase (NAMPT).

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$147	-	In Stock
5 mg	$247	-	In Stock
10 mg	$396	-	In Stock
25 mg	$787	-	In Stock
50 mg	$1,230	-	In Stock
100 mg	$1,630	-	In Stock
1 mL x 10 mM (in DMSO)	$272	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.31%

Color:White to Brown

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	OT-82 is a potent, selective, and orally active inhibitor of nicotinamide phosphoribosyltransferase (NAMPT).
Targets&IC50	NAMPT:
In vitro	METHODS: OT-82 (0.001-10 μM) was used to treat human cell lines derived from HP and non-HP cancers, and the IC50 values were determined. RESULTS: The average IC50 of OT-82 was significantly higher in non-HP cancer cells compared with HP cancer cells (13.03±2.94 nM vs 2.89±0.47 nM; p < 0.008). [1] METHODS: MV4-11 cells were treated with OT-82 (0.001-10 nM), and the changes in NAD were observed for 24 hours, and the changes in ATP were observed for 24 hours and 48 hours. RESULTS: OT-82 treatment resulted in a dose-dependent decrease in NAD and ATP concentrations in MV4-11 cells. OT-82 treatment of MV4-11 cells in vitro for 48 hours resulted in caspase-3 activation, an increase in the proportion of cells with sub-G1 DNA content, and mitochondrial membrane depolarization. OT-82 induced apoptosis and cell death by inhibiting NAMPT, leading to NAD and ATP depletion. [1]
In vivo	METHODS: OT-82 (25 or 50 mg/kg) was administered orally 6 days per week for 3 weeks. The therapeutic efficacy of OT-82 was tested in vivo using subcutaneous (SC) and systemic mouse xenograft models of HP malignancies. RESULTS: OT-82 caused a dose-dependent inhibition of SC growth of human AML-derived MV4-11 cells; the 25 mg/kg dose resulted in a 3-fold reduction in mean tumor volume at day 17 of treatment, while higher doses resulted in complete eradication of tumors within 10 days of treatment initiation. [1]
Synonyms	OT82, OT 82

Chemical Properties

Molecular Weight	424.47
Formula	C₂₆H₂₁FN₄O
Cas No.	1800487-55-1
Smiles	O=C(NCCCC=1C=NNC1)C=2C=CC(=C(C#CC3=CC=C(F)C=C3)C2)C=4C=CN=CC4
Relative Density.	1.31 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (188.47 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (7.77 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3559 mL	11.7794 mL	23.5588 mL	117.7940 mL
5 mM	0.4712 mL	2.3559 mL	4.7118 mL	23.5588 mL
10 mM	0.2356 mL	1.1779 mL	2.3559 mL	11.7794 mL
20 mM	0.1178 mL	0.5890 mL	1.1779 mL	5.8897 mL
50 mM	0.0471 mL	0.2356 mL	0.4712 mL	2.3559 mL
100 mM	0.0236 mL	0.1178 mL	0.2356 mL	1.1779 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc