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NST-628

Name: NST-628
Brand: TargetMol
SKU: T87041
Price: 199 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T87041Cas No. 3002056-30-3

Alias NST628, NST 628

NST-628 is a pan-RAF-MEK molecular glue that prevents RAF from phosphorylating and activating MEK, inhibiting the RAF-MEK signaling complex.

NST-628

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Purity: 98.86%

Catalog No. T87041Alias NST628, NST 628Cas No. 3002056-30-3

NST-628 is a pan-RAF-MEK molecular glue that prevents RAF from phosphorylating and activating MEK, inhibiting the RAF-MEK signaling complex.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$199	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.86%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	NST-628 is a pan-RAF-MEK molecular glue that prevents RAF from phosphorylating and activating MEK, inhibiting the RAF-MEK signaling complex.
In vitro	NST-628 demonstrates elevated antiproliferative activity in BRAF Class II/III mutant cell models compared to other RAF and MEK inhibitors, without promoting heterodimer formation of BRAF and CRAF. [1] NST-628 (4-100 nM; 48 h) induces early and late apoptosis in a dose-dependent manner and decreases live cell populations in NRAS mutant IPC-298 and SK-MEL-2, NF1 mutant MeWo, and KRAS mutant HCT116 cell lines. [1]
In vivo	NST-628 when administered orally (p.o.) at doses of 3 mg/kg and 5 mg/kg once daily (qd), or 1.5 mg/kg twice daily (b.i.d), significantly slowed tumor growth in models with KRAS and NRAS mutations. In the SK-MEL-2-luc model, it caused tumor regression. [1] Administered intragastrically (i.g.) at 0.3-3 mg/kg once daily for 18-20 days, NST-628 inhibited the RAS-MAPK pathway in mice in a dose-dependent manner, showing strong anti-tumor activity in the MeWo-luc model. At a dose of 2 mg/kg once daily for 26 days in an NCI-H23 KRASG12C-mutant lung adenocarcinoma model, NST-628 effectively slowed tumor growth and significantly inhibited the RAS-MAPK pathway. [1]
Synonyms	NST628, NST 628

Chemical Properties

Molecular Weight	488.46
Formula	C₂₂H₁₈F₂N₄O₅S
Cas No.	3002056-30-3
Smiles	O=C1OC=2C=C(OC3=NC=CC=C3F)C=CC2C(=C1CC=4C=CN=C(NS(=O)(=O)NC)C4F)C

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 80 mg/mL (163.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.76 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0473 mL	10.2363 mL	20.4725 mL	102.3625 mL
5 mM	0.4095 mL	2.0473 mL	4.0945 mL	20.4725 mL
10 mM	0.2047 mL	1.0236 mL	2.0473 mL	10.2363 mL
20 mM	0.1024 mL	0.5118 mL	1.0236 mL	5.1181 mL
50 mM	0.0409 mL	0.2047 mL	0.4095 mL	2.0473 mL
100 mM	0.0205 mL	0.1024 mL	0.2047 mL	1.0236 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc