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MMAF Hydrochloride

Name: MMAF Hydrochloride
Brand: TargetMol
SKU: T16121
Rating: 4.5 (1 reviews)

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Catalog No. T16121Cas No. 1415246-68-2

Alias Monomethylauristatin F Hydrochloride

MMAF Hydrochloride is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) such as Vorsetuzumab mafodotin and SGN-CD19A. MMAF Hydrochloride is an effective tubulin polymerization inhibitor and is used as an antitumor compound.

MMAF Hydrochloride

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Catalog No. T16121Alias Monomethylauristatin F HydrochlorideCas No. 1415246-68-2

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	Inquiry	1-2 weeks	1-2 weeks
50 mg	Inquiry	1-2 weeks	1-2 weeks
100 mg	Inquiry	1-2 weeks	1-2 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	MMAF Hydrochloride is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) such as Vorsetuzumab mafodotin and SGN-CD19A. MMAF Hydrochloride is an effective tubulin polymerization inhibitor and is used as an antitumor compound.
In vitro	MMAF suppresses anaplastic large cell lymphoma Karpas 299, breast carcinoma H3396, renal cell carcinoma 786-O, and Caki-1 cells. It has IC50s of 119, 105, 257, and 200 nM in vitro cytotoxicity assay[4].
In vivo	cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats, whereas the cAC10-L4-MMAF4 ADC is much less toxic, with MTDs of >150 mg/kg in mice and 90 mg/kg in rats. The maximum tolerated dose of MMAF (>16 mg/kg) in mice is much higher than that of MMAE (1 mg/kg) [4].
Synonyms	Monomethylauristatin F Hydrochloride

Chemical Properties

Molecular Weight	768.42
Formula	C₃₉H₆₆ClN₅O₈
Cas No.	1415246-68-2
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 27 mg/mL (35.14 mM), Sonication and heating are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (2.6 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3014 mL	6.5069 mL	13.0137 mL	65.0686 mL
5 mM	0.2603 mL	1.3014 mL	2.6027 mL	13.0137 mL
10 mM	0.1301 mL	0.6507 mL	1.3014 mL	6.5069 mL
20 mM	0.0651 mL	0.3253 mL	0.6507 mL	3.2534 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc