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ML-SA5

Name: ML-SA5
Brand: TargetMol
SKU: T60036
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T60036Cas No. 2418670-70-7

ML-SA5 is a MCOLN1 agonist that blocks autophagic flux by disrupting fusion between autophagosomes and lysosomes.

ML-SA5

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Purity: 99.16%

Catalog No. T60036Cas No. 2418670-70-7

ML-SA5 is a MCOLN1 agonist that blocks autophagic flux by disrupting fusion between autophagosomes and lysosomes.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$33	In Stock	In Stock
5 mg	$76	In Stock	In Stock
10 mg	$133	In Stock	In Stock
25 mg	$283	In Stock	In Stock
50 mg	$455	In Stock	In Stock
100 mg	$672	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$77	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.16%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	ML-SA5 is a MCOLN1 agonist that blocks autophagic flux by disrupting fusion between autophagosomes and lysosomes.
In vitro	METHODS: 0.05 or 0.1 μM ML-SA5 was used to treat HeLa cells 1 hour and 4 hours after the application of agonists, and the LC3-II levels in HeLa cells were observed. RESULTS ML-SA5 significantly increased the LC3-II levels. ML-SA5 regulates the LC3-II levels by stimulating the activity of MCOLN1 channels. [1] METHODS: Vero cells stably expressing GFP-Lamp1 were transfected with pStrep-mCherry-CvpE. Twenty hours after transfection, cells were treated with DMSO or ML-SA5 (30 μM) for 4 hours. Then, cells were fixed and nucleic acids were stained with DAPI. RESULTS ML-SA5 treatment completely blocked CvpE-induced cytoplasmic vacuolization. [2]
In vivo	METHODS: Patu 8988 t cells were injected into the subcutaneous tissue of nude mice, and ML-SA5 (5 μM) was injected into solid tumors daily. Tumor volume was measured daily, and immunohistochemistry and immunoblot analysis were performed after death of mice. RESULTS Administration of ML-SA5 profoundly promoted the survival of Patu 8988 t xenograft mice and inhibited tumor growth. [1]

Chemical Properties

Molecular Weight	457.99
Formula	C₁₉H₂₄ClN₃O₄S₂
Cas No.	2418670-70-7
Smiles	CN(C)S(=O)(=O)c1ccc(cc1)S(=O)(=O)Nc1cccc(Cl)c1N1CCCCC1
Relative Density.	1.395 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 122.5 mg/mL (267.47 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.37 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1835 mL	10.9173 mL	21.8345 mL	109.1727 mL
5 mM	0.4367 mL	2.1835 mL	4.3669 mL	21.8345 mL
10 mM	0.2183 mL	1.0917 mL	2.1835 mL	10.9173 mL
20 mM	0.1092 mL	0.5459 mL	1.0917 mL	5.4586 mL
50 mM	0.0437 mL	0.2183 mL	0.4367 mL	2.1835 mL
100 mM	0.0218 mL	0.1092 mL	0.2183 mL	1.0917 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc