Marrubiin is a natural terpenoid compound extracted from Marrubium vulgare that exhibits vasodilatory and anti-edematous effects, as well as anti-inflammatory, antioxidant, and antibacterial bioactivity.

Method: Marrubiin at various concentrations (0.0001–0.001 M) was preincubated with lipoxygenase (8000 U/mL) for 5 minutes. Sodium linoleate was then added as a substrate, and changes in absorbance were continuously monitored at 234 nm to calculate the inhibition rate.
Results: Marrubiin exhibited moderate inhibitory activity against LOX (IC50 = 173 μg/mL). [1]
Methods: Human umbilical vein endothelial cells (HUVECs) were pretreated with marrubiin (39 µM) for 24 hours, then stimulated with TNF-α (50 ng/mL) for 24 hours; GSH levels were measured using a glutathione assay kit.
Results: Pretreatment with marrubiin significantly increased GSH levels and antagonized the depleting effect of TNF-α, indicating that marrubiin protects endothelial cells by maintaining cellular antioxidant reserves.[2]

Methods: Inflammation was induced in C57BL/6 mice by intraperitoneal injection of carrageenan (1%, 250 μL). Marrubiin (1, 10, 20, 40 mg/kg) was administered intraperitoneally 1 hour prior to carrageenan stimulation, and the animals were sacrificed 4 hours after carrageenan stimulation.
Results: Marrubiin dose-dependently reduced the total number of inflammatory cells in the exudate, significantly decreased protein content and MPO activity in the exudate, and maintained or increased GPx activity, but had no significant effect on GSH levels. [1]

DMSO: 80 mg/mL (240.65 mM), Sonication is recommended.