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LM22A-4

Name: LM22A-4
Brand: TargetMol
SKU: T7568
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7568Cas No. 37988-18-4

LM22A-4 is a brain-derived neurotrophic factor (BDNF) mimetic and agonist of the receptor tropomyosin-related kinase B (TrkB; IC50 : 47 nM in a fluorescence anisotropy assay)

LM22A-4

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Purity: 99.56%

Catalog No. T7568Cas No. 37988-18-4

LM22A-4 is a brain-derived neurotrophic factor (BDNF) mimetic and agonist of the receptor tropomyosin-related kinase B (TrkB; IC50 : 47 nM in a fluorescence anisotropy assay)

Pack Size	Price	Availability
5 mg	$39	In Stock
10 mg	$72	In Stock
25 mg	$155	In Stock
50 mg	$272	In Stock
100 mg	$478	In Stock
500 mg	$1,050	In Stock
1 mL x 10 mM (in DMSO)	$43	In Stock

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Purity:99.56%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	LM22A-4 is a brain-derived neurotrophic factor (BDNF) mimetic and agonist of the receptor tropomyosin-related kinase B (TrkB; IC50 : 47 nM in a fluorescence anisotropy assay)
Targets&IC50	TrkB:47 nM
In vitro	In vitro, LM22A-4 increases survival of hippocampal neurons via activation of TrkB as well as Akt and ERK downstream pro-survival signaling pathways[1].
In vivo	Treatment of Het mice with LM22A-4 for 4 weeks rescued wild-type levels of TrkB phosphorylation in the medulla and pons and restored wild-type breathing frequency[1].
Animal Research	Wt and Het littermates were divided into 4 groups: Wt vehicle-treated (100 μl of 0.9% NaCl, i.p., b.i.d.), Wt drug-treated (50 mg/kg LM22A-4 in 0.9% NaCl, i.p., b.i.d.), Het vehicle-treated (100 μl of 0.9% NaCl, i.p., b.i.d.), and Het drug-treated (50 mg/kg LM22A-4 in 0.9% NaCl, i.p., b.i.d.).?Each mouse was treated from 8 to 13 weeks of age.?Whole-body plethysmography was performed during week 12 and animals were subsequently killed and tissues harvested for TrkB Western blots and BDNF ELISA during week[1].

Chemical Properties

Molecular Weight	339.34
Formula	C₁₅H₂₁N₃O₆
Cas No.	37988-18-4
Smiles	OCCNC(=O)c1cc(cc(c1)C(=O)NCCO)C(=O)NCCO
Relative Density.	no data available
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 50 mg/mL (147.34 mM), Sonication is recommended.

DMSO: 45 mg/mL (132.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.89 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	2.9469 mL	14.7345 mL	29.4690 mL	147.3448 mL
5 mM	0.5894 mL	2.9469 mL	5.8938 mL	29.4690 mL
10 mM	0.2947 mL	1.4734 mL	2.9469 mL	14.7345 mL
20 mM	0.1473 mL	0.7367 mL	1.4734 mL	7.3672 mL
50 mM	0.0589 mL	0.2947 mL	0.5894 mL	2.9469 mL
100 mM	0.0295 mL	0.1473 mL	0.2947 mL	1.4734 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc