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IST5-002

Name: IST5-002
Brand: TargetMol
SKU: T62496
Rating: 4.5 (1 reviews)

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Catalog No. T62496Cas No. 13484-66-7

Alias N6-Benzyladenosine-5'-phosphate, IST5002

IST5-002 (N6-Benzyladenosine-5'-phosphate) is a selective Stat5a/b inhibitor with anticancer activity that inhibits the transcriptional activity of Stat5a/b and induces apoptosis and death of prostate cancer cells and chronic myelogenous leukemia (CML) cells, which can be used for cancer research.

IST5-002

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Purity: 99.36%

Catalog No. T62496Alias N6-Benzyladenosine-5'-phosphate, IST5002Cas No. 13484-66-7

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,520	6-8 weeks	6-8 weeks
50 mg	$1,980	6-8 weeks	6-8 weeks
100 mg	$2,500	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.36%

Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	IST5-002 (N6-Benzyladenosine-5'-phosphate) is a selective Stat5a/b inhibitor with anticancer activity that inhibits the transcriptional activity of Stat5a/b and induces apoptosis and death of prostate cancer cells and chronic myelogenous leukemia (CML) cells, which can be used for cancer research.
Targets&IC50	STAT5a:1.5 μM, STAT5b:3.5 μM
In vitro	IST5-002 is a potent Stat5a/b inhibitor that selectively blocks the transcriptional activity of Stat5a and Stat5b with IC50 values of 1.5 μM (Stat5a) and 3.5 μM (Stat5b), respectively.IST5-002 showed significant inhibitory effects on both Stat5a and Stat5b in a variety of cellular assays. Under different dosage and time conditions, IST5-002 was able to inhibit Bcr-Abl-induced Stat5a/b phosphorylation in K562 cells (0-40 μM, 3 h), Stat5a/b phosphorylation in T47D cells (5-100 μM, 2 h), and Stat5 dimerization and nuclear translocation in PC-3 cells, as well as inhibit the Stat5 target gene and DNA binding in COS-7 cells. In CWR22Rv1 and LNCaP cells, IST5-002 (2-50 μM, 48 h) significantly reduced the expression of Stat5a/b target genes such as Bcl-xL and cyclin D1. In addition, IST5-002 inhibited the growth of human prostate cancer cells by inducing apoptosis at high concentrations (3.1-50 μM, 72 hours) and triggered epithelial cell death in patient-derived prostate cancer samples cultured in vitro (25-100 μM, 7 days). IST5-002 (5 μM, 24-72 h) similarly inhibited Stat5a/b phosphorylation and induced apoptosis in Imatinib-sensitive and -resistant chronic granulocytic leukemia (CML) cells. [1]
In vivo	Tumor growth was effectively inhibited in a prostate cancer xenograft model by intraperitoneal injection of 25-100 mg/kg IST5-002 once daily for 10 days. [1]
Synonyms	N6-Benzyladenosine-5'-phosphate, IST5002

Chemical Properties

Molecular Weight	437.34
Formula	C₁₇H₂₀N₅O₇P
Cas No.	13484-66-7
Smiles	O[C@H]1[C@H](N2C=3C(N=C2)=C(NCC4=CC=CC=C4)N=CN3)O[C@H](COP(=O)(O)O)[C@H]1O

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 100 mg/mL (228.66 mM), Sonication is recommended.

DMSO: 100 mg/mL (228.66 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2866 mL	11.4328 mL	22.8655 mL	114.3275 mL
5 mM	0.4573 mL	2.2866 mL	4.5731 mL	22.8655 mL
10 mM	0.2287 mL	1.1433 mL	2.2866 mL	11.4328 mL
20 mM	0.1143 mL	0.5716 mL	1.1433 mL	5.7164 mL
50 mM	0.0457 mL	0.2287 mL	0.4573 mL	2.2866 mL
100 mM	0.0229 mL	0.1143 mL	0.2287 mL	1.1433 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc