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Furamidine dihydrochloride

Name: Furamidine dihydrochloride
Brand: TargetMol
SKU: T27395
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T27395Cas No. 55368-40-6

Alias NSC305831 dihydrochloride, NSC 305831 dihydrochloride, DB75 dihydrochloride

Furamidine dihydrochloride (DB75 dihydrochloride) is a cell-permeable, selective small molecule inhibitor of protein arginine methyltransferase 1 (PRMT1) that also binds preferentially to strings of AT base pair sequences within the minor groove of DNA; it targets the PRMT1 enzyme active site primarily through a competitive mechanism with the protein substrate and is noncompetitive toward the cofactor, ultimately leading to the blockade of cell proliferation in various leukemia cell lines irrespective of their genetic lesions.

Furamidine dihydrochloride

🥰Excellent

Purity: 98.16%

Catalog No. T27395Alias NSC305831 dihydrochloride, NSC 305831 dihydrochloride, DB75 dihydrochlorideCas No. 55368-40-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$34	-	In Stock
5 mg	$77	-	In Stock
10 mg	$135	-	In Stock
25 mg	$293	-	In Stock
50 mg	$519	-	In Stock
100 mg	$893	-	In Stock
200 mg	$1,180	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.16%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Furamidine dihydrochloride (DB75 dihydrochloride) is a cell-permeable, selective small molecule inhibitor of protein arginine methyltransferase 1 (PRMT1) that also binds preferentially to strings of AT base pair sequences within the minor groove of DNA; it targets the PRMT1 enzyme active site primarily through a competitive mechanism with the protein substrate and is noncompetitive toward the cofactor, ultimately leading to the blockade of cell proliferation in various leukemia cell lines irrespective of their genetic lesions.
Targets&IC50	PRMT4:>400 µM (IC50), PRMT5:166 µM (IC50), PRMT6:283 µM (IC50), Protein arginine methyltransferase 1 (PRMT1):9.4 μM (IC50)
In vitro	Furamidine dihydrochloride (20 μM, 72 hours) treatment of leukemia cell lines inhibited cell growth in most leukemia cell lines except HEL cells harboring the JAK2V617F mutation.[6]
In vivo	Furamidine dihydrochloride (1 mg/kg, intraperitoneal injection, 3 times a week, repeated every 4 weeks for 34 weeks) was used to treat female NZB/NZW mice and was found to suppress proteinuria and prolong the survival of lupus-prone NZB/NZW mice when combined with irinotecan. [5]
Synonyms	NSC305831 dihydrochloride, NSC 305831 dihydrochloride, DB75 dihydrochloride

Chemical Properties

Molecular Weight	377.27
Formula	C₁₈H₁₈Cl₂N₄O
Cas No.	55368-40-6
Smiles	N=C(C1=CC=C(C2=CC=C(C3=CC=C(C(N)=N)C=C3)O2)C=C1)N.Cl.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 10 mg/mL (26.51 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.65 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6506 mL	13.2531 mL	26.5062 mL	132.5311 mL
5 mM	0.5301 mL	2.6506 mL	5.3012 mL	26.5062 mL
10 mM	0.2651 mL	1.3253 mL	2.6506 mL	13.2531 mL
20 mM	0.1325 mL	0.6627 mL	1.3253 mL	6.6266 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc