EFdA-TP tetraammonium is an influential nucleoside reverse transcriptase (RT) inhibitor that effectively prevents RT-mediated DNA synthesis by acting as an immediate or delayed chain terminator (ICT or DCT). Additionally, EFdA-TP tetraammonium exhibits multiple mechanisms of inhibiting HIV-1 RT[1].

EFdA-TP tetraammonium, at concentrations ranging from 0.05 to 10 μM and applied for 15 minutes, serves as an effective inhibitor of reverse transcriptase (RT)-catalyzed DNA synthesis, functioning through immediate or delayed chain termination mechanisms[1]. It acts as a translocation-defective RT inhibitor, significantly reducing the pace of DNA synthesis and essentially serving as an immediate chain terminator. Additionally, EFdA-TP tetraammonium has the capability to operate as a delayed chain terminator, permitting the incorporation of one more dNTP prior to halting DNA synthesis[1].