Direct Violet 1 (CCRIS 2413) is an agent of dye.

TNF-R1-TNFα binding:2.11 µM (IC50), SARS-CoV-2-S-RBD binding to ACE2:1.47 µM (IC50), SARS-CoV-S1S2 binding to ACE2:2.63 µM (IC50)

1. Solution preparation
1. Solution preparation: Dissolve Direct Violet 1 in an appropriate amount of water, usually at a concentration of 0.5-2% (adjust according to the desired depth of dyeing).
2. Working solution preparation: Dissolve Direct Violet 1 in an appropriate amount of solvent (such as DMSO or water) to the desired concentration, usually 1-10 μM.
2. Textile dye application
1. Immerse the fabric in the dye solution and use conventional textile dyeing procedures, usually heating to 60-90°C, and the dyeing time is adjustable (usually 30-60 minutes).
2. During the dyeing process, pH adjustment and temperature control can be used to optimize dye adsorption.
3. Washing and fixing:
1) After dyeing, wash the fabric with water to remove excess dye.
2) A fixing agent can be used as needed to improve the durability and color stability of the dye.
III. PPI Inhibition of SARS-CoV-2 Spike Protein and ACE2 Receptor
1. Mix SARS-CoV-2 spike protein and ACE2 receptor in vitro and set different concentrations of Direct Violet 1.
2. Measure the inhibitory effect of Direct Violet 1 on protein-protein interaction using surface plasmon resonance (SPR), fluorescence resonance energy transfer (FRET) or other interaction detection techniques.
3. IC50 determination: By comparing the effects of different concentrations of Direct Violet 1, calculate its IC50 value (1.47-2.63 μM) for inhibiting the interaction between SARS-CoV-2 spike protein and ACE2 receptor.
4. Application:
1) As a tool to study the interaction between SARS-CoV-2 spike protein and ACE2 receptor.
2) As a potential candidate molecule for anti-SARS-CoV-2 drugs.