DFHBI-1T is an RNA aptamer-activated fluorescent probe that transmits through cell membranes.DFHBI-1T can be used for dynamic localization imaging of RNA in living cells.

DFHBI-1T (20 μM; 10 minutes) enhances the fluorescence expression of (CGG)60-Spinach2 in COS7 cells compared to DFHBI (20 μM)[2]. The fluorescence characteristics are specified as follows: Broccoli-DFHBI-1T with ex/em=472 nm/507 nm, and Spinach2-DFHBI-1T with ex/em=482 nm/505 nm[1].

I. Neuronal morphological studies:
1. Tissue preparation: SR101 can be used on brain slices or living tissues. Incubate the tissue with SR101 solution for 10-30 minutes.
2. Imaging: After incubation, observe the tissue using a fluorescence microscope. SR101 emits red fluorescence at 605 nm when excited at 586 nm, allowing the structure of neurons to be observed.
II. Astrocyte labeling
1. Cell incubation: Incubate SR101 with cultured cells or brain slices for about 10-20 minutes. Astrocytes preferentially absorb the dye.
2. Imaging: Using a fluorescence microscope, the red fluorescence of SR101 can distinguish astrocytes from other cell types. It is often used in combination with other neuronal markers for colocalization studies.
III. In vivo studies
1. Injection: SR101 can label astrocytes in vivo by intravenous injection or cerebrospinal fluid injection.
2. In vivo imaging: Use in vivo fluorescence microscopy or multiphoton microscopy to image the labeled cells.
IV. Slice imaging
1. Tissue incubation: Incubate brain slices with SR101 solution for 10-20 minutes to allow the dye to penetrate into the cells.
2. Imaging: After incubation, observe the tissue under a fluorescence microscope for detailed structural and cellular analysis.