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D159687

Name: D159687
Brand: TargetMol
SKU: T5474
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5474Cas No. 1155877-97-6

Alias D 159687

D159687 is a selective PDE4D inhibitor,had a procognitive profile as it improved memory in the novel object recognition test but had no antidepressant or anxiolytic benefit.

D159687

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Purity: 97.57%

Catalog No. T5474Alias D 159687Cas No. 1155877-97-6

D159687 is a selective PDE4D inhibitor,had a procognitive profile as it improved memory in the novel object recognition test but had no antidepressant or anxiolytic benefit.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$30	In Stock	In Stock
10 mg	$46	In Stock	In Stock
25 mg	$98	In Stock	In Stock
50 mg	$158	In Stock	In Stock
100 mg	$253	In Stock	In Stock
200 mg	$376	-	In Stock
1 mL x 10 mM (in DMSO)	$31	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.57%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	D159687 is a selective PDE4D inhibitor,had a procognitive profile as it improved memory in the novel object recognition test but had no antidepressant or anxiolytic benefit.
In vitro	D159687 induced a transient increase in CREB phosphorylation which peaked at 6 hrs after treatment ( F (3, 20) = 3.731, P = 0.0425). CREB phosphorylation was optimal at 1 μM ( F (3, 20) = 4.194, P = 0.0302).
Cell Research	cAMP concentrations were measured using the enzyme-linked immunosorbent assay.?On the day of treatment, the attached HT-22 cells were washed with warm phosphate buffer saline and then incubated for 10 min with PBS supplemented with various concentrations of D159687 and further for 10 min with 10 nM isoproterenol in the system52.?After incubation, PBS containing the drugs was removed and the plates were let dry roughly before 200 μl HCl (0.1 M) was added to each well to lyse the cells.?Cell lysates were collected into Eppendorf tubes and centrifuged at 800 g for 15 min. Supernatants were collected for immediate assay or stored frozen for assay later using the cAMP complete ELISA kit according to the assay protocol.
Synonyms	D 159687

Chemical Properties

Molecular Weight	366.84
Formula	C₂₁H₁₉ClN₂O₂
Cas No.	1155877-97-6
Smiles	COc1ccc(Cc2ccc(NC(N)=O)cc2)cc1-c1cccc(Cl)c1
Relative Density.	1.263 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (408.9 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (10.9 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7260 mL	13.6299 mL	27.2598 mL	136.2992 mL
5 mM	0.5452 mL	2.7260 mL	5.4520 mL	27.2598 mL
10 mM	0.2726 mL	1.3630 mL	2.7260 mL	13.6299 mL
20 mM	0.1363 mL	0.6815 mL	1.3630 mL	6.8150 mL
50 mM	0.0545 mL	0.2726 mL	0.5452 mL	2.7260 mL
100 mM	0.0273 mL	0.1363 mL	0.2726 mL	1.3630 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc