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BTB09089

Name: BTB09089
Brand: TargetMol
SKU: T67892
Price: 161 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: GPR65 agonist (BTB09089)) Copy Product Info

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Synonyms: GPR65 agonist (BTB09089)

Catalog No. T67892 Copy Product Info

Purity: 99.78%

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BTB09089 (GPR65 agonist ) is a T cell death-associated gene 8 (TDAG8/GPR65)-specific agonist.BTB09089 induces TDAG8 expression and regulates cytokine production in T cells and macrophages.

Cas No. 245728-44-3

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Pack Size	Price	USA Stock	Global Stock
1 mg	$65	In Stock	In Stock
5 mg	$126	In Stock	In Stock
10 mg	Inquiry	In Stock	In Stock
25 mg	Inquiry	In Stock	In Stock
50 mg	Inquiry	Inquiry	Inquiry
100 mg	Inquiry	Inquiry	Inquiry
500 mg	Inquiry	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$161	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:99.78%

Appearance:Solid

Color:Orange

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	BTB09089 (GPR65 agonist ) is a T cell death-associated gene 8 (TDAG8/GPR65)-specific agonist.BTB09089 induces TDAG8 expression and regulates cytokine production in T cells and macrophages.
In vitro	BTB09089 (0-18 μM; 30 min) significantly increases cAMP accumulation in HEK293 cells expressing hTDAG8 and mTDAG8, but not in control HEK293 cells. It does not enhance cAMP accumulation in HEK293 cells expressing hGPR4 or inositol phosphate accumulation in HEK293 cells expressing hOGR1.[1] BTB09089 (0-18 μM; 30 min) enhances cAMP accumulation dose-dependently at pH 7.0-7.9, but not at pH 6.5.[1] BTB09089 (1-5 μM; 20 h) inhibits IL-2 production in WT mouse splenocytes dose-dependently, without affecting IL-2 production in TDAG8 KO mice or cell viability.[1] BTB09089 (1-5 μM; 18 h) inhibits TNF-α and IL-6 production stimulated by LPS while enhancing IL-10 production in TG-PEC induced by LPS stimulation with sulfated ester.[1]
In vivo	BTB09089 (5-20 μM, 8 μL; i.c.v.; administered 6 hours prior to MCAO) reduces cerebral ischemia-reperfusion injury in rats.[2]
Synonyms	GPR65 agonist (BTB09089)

Chemical Properties

Molecular Weight	387.31
Formula	C₁₄H₁₂Cl₂N₄OS₂
Cas No.	245728-44-3
Smiles	CN1C2=C(SC(SCC3=C(Cl)C=C(Cl)C=C3)=N1)C(=O)N(C)N=C2
Relative Density.	1.57 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 14.7 mg/mL (37.95 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn oil: < 1.47 mg/mL (3.8 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% Saline: < 1.47 mg/mL (3.8 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: < 1.47 mg/mL (3.8 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% (20% SBE-β-CD in Saline): < 1.47 mg/mL (3.8 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5819 mL	12.9096 mL	25.8191 mL	129.0956 mL
5 mM	0.5164 mL	2.5819 mL	5.1638 mL	25.8191 mL
10 mM	0.2582 mL	1.2910 mL	2.5819 mL	12.9096 mL
20 mM	0.1291 mL	0.6455 mL	1.2910 mL	6.4548 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc