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Bronopol

Name: Bronopol
Brand: TargetMol
SKU: T0880
Rating: 4.5 (1 reviews)

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Catalog No. T0880Cas No. 52-51-7

Alias BNPK, BNPD

Bronopol (BNPD) is an antimicrobial agent with low mammalian toxicity (at in-use levels) and high activity against bacteria, especially the troublesome Gram-negative species.

Bronopol

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Purity: 99.85%

Catalog No. T0880Alias BNPK, BNPDCas No. 52-51-7

Bronopol (BNPD) is an antimicrobial agent with low mammalian toxicity (at in-use levels) and high activity against bacteria, especially the troublesome Gram-negative species.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
50 g	$33	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.85%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bronopol AI Summary

Bronopol exhibits a diverse range of bioactivities. It shows inhibitory activity against targets such as Lamin A splicing, ROR gamma transcriptional activity, Bacillus subtilis Sfp phosphopantetheinyl transferase, GCN5L2, T. brucei phosphofructokinase, Histone Lysine Methyltransferase G9a, Glutaminase (GLS), Trypanosoma brucei, and human tyrosyl-DNA phosphodiesterase 1 (TDP1). It acts as an agonist towards retinoid X receptor alpha signaling, thyroid hormone receptor beta signaling, the antioxidant response element (ARE) signaling pathway, and Nrf2-dependent transcriptional activity. Furthermore, it exhibits antagonistic activity against vitamin D receptor signaling and retinoid X receptor alpha signaling. Bronopol induces DNA re-replication in MCF 10a normal breast cells and SW480 colon adenocarcinoma cells. It also affects various cellular responses, such as Schwann cell viability, firefly luciferase enzyme activity, NPC, nuclear beta-catenin stimulation in WNT3A conditioned C2C12 cells, and VEGF-stimulated ADSC/ECFC co-culture. Significantly, it does not inhibit human BSEP, MRP2, MRP3, or MRP4, with IC50 values for these activities being greater than 133,000.0 nM. It demonstrates potent antiviral activities, notably inhibiting SARS-CoV-2-induced cytotoxicity in Caco-2 cells with an inhibition rate of -9.83% at a concentration of 10 µM after 48 hours, and inhibiting SARS-CoV-2 3CL-Pro protease with a 100.41% inhibition at 20 µM and an IC50 value of 420.0 nM. In contrast, its inhibition of the MERS 3CL protease is less significant, showing only 14.0% inhibition at 10 µM and an IC50 value greater than 10,000.0 nM. Additionally, Bronopol shows significant inhibitory activity for human HDAC6 enzyme with an inhibition rate of 135.55% using a commercial peptide substrate, and 8.53% using a custom peptide substrate. These findings suggest potential bioactivities related to HDAC6 inhibition..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Bronopol (BNPD) is an antimicrobial agent with low mammalian toxicity (at in-use levels) and high activity against bacteria, especially the troublesome Gram-negative species.
Synonyms	BNPK, BNPD

Chemical Properties

Molecular Weight	199.99
Formula	C₃H₆BrNO₄
Cas No.	52-51-7
Smiles	OCC(Br)(CO)[N+]([O-])=O
Relative Density.	2.0002 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 36 mg/mL (180.01 mM), Sonication is recommended.

DMSO: 245 mg/mL (1225.06 mM), Sonication is recommended.

Ethanol: 37 mg/mL (185.01 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (10 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.0003 mL	25.0013 mL	50.0025 mL	250.0125 mL
5 mM	1.0001 mL	5.0003 mL	10.0005 mL	50.0025 mL
10 mM	0.5000 mL	2.5001 mL	5.0003 mL	25.0013 mL
20 mM	0.2500 mL	1.2501 mL	2.5001 mL	12.5006 mL
50 mM	0.1000 mL	0.5000 mL	1.0001 mL	5.0003 mL
100 mM	0.0500 mL	0.2500 mL	0.5000 mL	2.5001 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc