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Protocatechuic acid

Catalog No. T0562   CAS 99-50-3
Synonyms: 3, 4-Dihydroxybenzoic acid, Protocatechuate, 3,4-Dihydroxybenzoic acid

Protocatechuic acid (3, 4-Dihydroxybenzoic acid) (3, 4-dihydroxybenzoic acid) is a natural phenolic compound found in many edible and medicinal plants. Recent studies indicate that it could be used as a protective agent against cardiovascular diseases and neoplasms. The mechanism of its action is mostly associated with antioxidant activity, including inhibition of generation as well as scavenging of free radicals and up-regulating enzymes which participate in their neutralization.

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Protocatechuic acid Chemical Structure
Protocatechuic acid, CAS 99-50-3
Pack Size Availability Price/USD Quantity
200 mg In stock $ 48.00
1 mL * 10 mM (in DMSO) In stock $ 54.00
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Purity: 99.85%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Protocatechuic acid (3, 4-Dihydroxybenzoic acid) (3, 4-dihydroxybenzoic acid) is a natural phenolic compound found in many edible and medicinal plants. Recent studies indicate that it could be used as a protective agent against cardiovascular diseases and neoplasms. The mechanism of its action is mostly associated with antioxidant activity, including inhibition of generation as well as scavenging of free radicals and up-regulating enzymes which participate in their neutralization.
In vitro Protocatechuic acid inhibits the aggregation of Aβ and αS and destabilizes their preformed fibrils that prevent the death of PC12 cells triggered by Aβ- and αS-induced toxicity[3].
In vivo Protocatechuic acid can prevent stress-induced immobility time in forced swim test without altering locomotor activity in mice. In addition, Protocatechuic acid treatment attenuates the elevation of serum corticosterone, lipid peroxidation and restores enzymatic antioxidants in cerebral cortex and hippocampus in ARS mice[1]. Rat administered cadmium and treated with prostigmine and doses of Protocatechuic acid (10−20 mg/kg) has significantly reduced BChE activity. Cadmium and either prostigmine or Protocatechuic acid (10−20 mg/kg) treated rats shows a marked reduction in MDA level[2].
Kinase Assay AChE activity investigation is carried out in a reaction mixture containing 50 μL of tissue homogenate, 50 μL of 5, 5'-dithiobis-(2-nitrobenzoic) acid (DTNB), 1175 μL of 0.1 M phosphate-buffered solution, pH 8.0. After incubation for 20 min at 25°C, 25 μL of acetylthiocholine iodide solution is added as the substrate. The AChE activity is determined as changes in absorbance reading at 412 nm for 3 min at 25°C and using a UV/Visible spectrophotometer.
Cell Research Protocatechuic acid is dissolved in DMSO. Dilutions of Protocatechuic acid (2, 5, 10, 20, 50, and 100 μM) are prepared from stock solutions, with serum-free culture medium. Equal volumes of each solution are mixed with Aβ1-42 (10 μM), then incubated for 24 h on a thermoblock, with continuous agitation, and then exposed to PC12 cells for 24 h to test whether Protocatechuic acid can prevent cell death triggered by Aβ. Cell viability is determined by MTT reduction assay. Cells are treated with 200 μL per well of MTT solution (final concentration, 0.5 mg/mL in DMEM-Glutamax medium) for 3 h, at 37°C, with 5% CO2. The dark blue formazan crystals that formed are solubilized with 100 μL per well of DMSO, for 30 min. Absorbance is measured at 540 nm, with a microplate reader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of control cells at 100%.
Source
Synonyms 3, 4-Dihydroxybenzoic acid, Protocatechuate, 3,4-Dihydroxybenzoic acid
Molecular Weight 154.12
Formula C7H6O4
CAS No. 99-50-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 55 mg/mL (356.86 mM)

TargetMolReferences and Literature

1. Thakare VN, et al. Attenuation of acute restraint stress-induced depressive like behavior and hippocampal alterations with protocatechuic acid treatment in mice. Metab Brain Dis. 2016 Oct 26. 2. Adefegha SA, et al. Alterations of Na+/K+-ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomed Pharmacother. 2016 Oct;83:559-568. 3. Hornedo-Ortega R , álvarez-Fernández, María Antonia, Cerezo, Ana Belén, et al. Protocatechuic acid: inhibition of fibril formation, destabilization of pre-formed fibrils of Amyloid-β and α-Synuclein, and neuroprotection[J]. Journal of Agricultural and Food Chemistry, 2016:acs.jafc.6b03217. 4. Jang S A, Hwang Y H, Kim T, et al. Anti-Osteoporotic and Anti-Adipogenic Effects of the Water Extract of Drynaria roosii Nakaike in Ovariectomized Mice Fed a High-Fat Diet[J]. Molecules. 2019, 24(17): 3051. 5. Dong Q, Li Y, Liu G, et al. High oxygen treatments enhance the contents of phenolic compound and ganoderic acid, antioxidant and DNA damage protective activities of Ganoderma lingzhi fruiting body[J]. Frontiers in Microbiology. 2019, 10: 2363. 6. Chen Q, Sun D, Fang T, et al. In vitro allelopathic effects of compounds from Cerbera manghas L. on three Dinophyta species responsible for harmful common red tides. Science of The Total Environment. 2020: 142253.

TargetMolCitations

1. Chen Z, Yuan Y, Yang D, et al. Antiviral activities of Polygonum Perfoliatum L. extract and related phenolic acid constituents against hepatitis B virus. Journal of Medical Virology. 2022 2. Dong Q, Li Y, Liu G, et al. High oxygen treatments enhance the contents of phenolic compound and ganoderic acid, antioxidant and DNA damage protective activities of Ganoderma lingzhi fruiting body. Frontiers in Microbiology. 2019, 10: 2363 3. Tsang C C, Tang J Y M, Ye H, et al. Rare/cryptic Aspergillus species infections and importance of antifungal susceptibility testing. Mycoses. 2020, 63(12): 1283-1298 4. Jang S A, Hwang Y H, Kim T, et al. Anti-Osteoporotic and Anti-Adipogenic Effects of the Water Extract of Drynaria roosii Nakaike in Ovariectomized Mice Fed a High-Fat Diet. Molecules. 2019, 24(17): 3051

Related compound libraries

This product is contained In the following compound libraries:
Microbial Natural Product Library Tobacco Monomer Library Miao medicine Compound Library Selected Plant-Sourced Compound Library Ferroptosis Compound Library Human Metabolite Library Food as Medicine Compound Library Traditional Chinese Medicine Monomer Library Traditional Mongolian Medicine Compound Library Anti-COVID-19 Traditional Chinese Medicine Compound Library

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Keywords

Protocatechuic acid 99-50-3 Others Endogenous Metabolite 3, 4-Dihydroxybenzoic acid Inhibitor Protocatechuate inhibit 3,4-Dihydroxybenzoic acid inhibitor

 

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