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MS-444

Catalog No. T16145 CAS 150045-18-4

Synonyms: BE-34776

MS-444 suppresses the activity of purified smooth muscle myosin light chain kinase (MLCK) (IC50: 10 μM).

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MS-444, CAS 150045-18-4

Pack Size	Availability	Price/USD
25 mg	8-10 weeks	$ 1,820.00
50 mg	8-10 weeks	$ 2,380.00
100 mg	8-10 weeks	$ 3,100.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

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Description	MS-444 suppresses the activity of purified smooth muscle myosin light chain kinase (MLCK) (IC50: 10 μM).
Targets&IC50	Myosin:10 μM
In vitro	MS-444 is a small molecule RNA-binding protein HuR (ELAVL1) inhibitor. Colorectal cancer (CRC) cells that show HuR overexpression are treated with MS-444 (1-100 μM) for 48 hr (IC50s: 10.98±1.76 μM, 12.84±2.10 μM, 5.60±0.90 μM, 14.21±2.11 μM, and 10.98±1.24 μM for HCT116, HCA-7, RKO, HT-29, and SW480 cells, respectively). Contrasting effects are observed using non-transformed small intestinal (RIE-1 (IC50=40.70±3.53 μM)) and colonic (YAMC (IC50=28.16±3.23 μM)) epithelial cells. Both non-transformed cell lines are ~3- to 4-fold less responsive to MS-444-mediated growth inhibition, with IC50 values of 40.70 μM and 28.16 μM (P<0.05). Growth inhibition is observed in all CRC lines with IC50 values ranging from 5.60 μM to 14.21 μM with observable effects seen at 10 μM MS-444 [2].
In vivo	Mice bearing HCT116 cell xenografts receive IP injections of MS-444 (25 mg/kg bw) or vehicle every 48 hr to test the effects of MS-444 on CRC cell growth in vivo. Over the experiment course, mice do not show any adverse effects and maintained similar weights. Anti-tumor effects of MS-444 are observed with an approximately 1.7-fold reduction in tumor size. Mice treated with MS-444 shows a marked 2- to 3-fold reduction in microvessel density (MVD), showing the anti-angiogenic potential of MS-444[2].

Synonyms	BE-34776
Molecular Weight	230.22
Formula	C13H10O4
CAS No.	150045-18-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Satoshi Nakanishi. et al. MS-444, a new inhibitor of myosin light chain kinase from 2. Fernando F. Blanco.et al, Impact of HuR inhibition by the small molecule MS-444 on colorectal cancer cell tumorigenesis. Oncotarget. 2016 Nov 8; 7(45): 74043-74058.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

MS-444 150045-18-4 Others BE 34776 BE34776 BE-34776 MS444 MS 444 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.