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FIN56

Catalog No. T4066   CAS 1083162-61-1

FIN56 is a specific inducer of ferroptosis.

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FIN56 Chemical Structure
FIN56, CAS 1083162-61-1
Pack Size Availability Price/USD Quantity
2 mg In stock $ 35.00
5 mg In stock $ 57.00
10 mg In stock $ 91.00
25 mg In stock $ 203.00
50 mg In stock $ 379.00
100 mg In stock $ 563.00
500 mg In stock $ 1,230.00
1 mL * 10 mM (in DMSO) In stock $ 65.00
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Purity: 99.78%
Purity: 97.86%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description FIN56 is a specific inducer of ferroptosis.
In vitro FIN56 triggers ferroptosis through a mechanism involving the regulation of GPX4 protein abundance. FIN56 causes the loss of GPX4 activity in cell lysates. FIN56-induced cell death is suppressed by GFP-GPX4 fusion protein overexpression.
In vivo NA
Cell Research 1000 cells/36 μL are seeded in each well in 384-well plates. Lethal compounds are dissolved and a 2-fold, 12-point dilution series are prepared in DMSO. Compound solutions are further diluted with media at 1:25 and 4 μL/well of the diluted solutions are added to cell cultures immediately after cells are seeded. When ferroptosis inhibitors (100 μM α-tocopherol, 152 μM deferoxamine, or 10 μM U-0126) are co-treated with lethal inducers, they are supplemented to cell culture at the same time as lethal compounds are added, and the cells are incubated for 24 hrs. When other cell death modulating compounds (100 nM sodium selenite, 1 μM cerivastatin, 100 μg/mL mevalonic acid) are co-treated, they are first supplemented to cell culture for 24 hrs before lethal compounds are added to cell culture and further incubated for 24 hrs at 37°C under 5% CO2. On the day of the viability measurement, 10 μL/well of 50% Alamar Blue diluted in media is added and further incubated at 37°C for 6 hrs. Fluorescence intensity (ex/em: 530/590) is measured with a Victor 3 plate reader and the normalized viability is calculated by VL = (IL-I0)/(IV-I0), where VL, I0, IV, and IL are the normalized viability, raw fluorescence intensities from the wells containing media, cells treated with a vehicle (negative control), and cells with the lethal compound (L), respectively. When the effect of a chemical modulator (M) on L is calculated, we instead used the equation: VL|M = (IM, L-I0)/(IM, V-I0), where VL|M, IM, L and IM, V are the normalized viability, and fluorescence intensity from cells treated with M and V, and from cells with M and L. respectively. The viability is typically measured in biological triplicates unless otherwise specified. A representative dose-response curve, the mean and standard error of normalized viability from one replicate are plotted.
Molecular Weight 517.66
Formula C25H31N3O5S2
CAS No. 1083162-61-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 51.8 mg/mL(100 mM)

TargetMolReferences and Literature

1. Shimada K, et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016 Jul;12(7):497-503. 2. Yan B, Ai Y, Sun Q, et al. Membrane Damage during Ferroptosis Is Caused by Oxidation of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1[J]. Molecular Cell. 2020

TargetMolCitations

1. Yan B, Ai Y, Sun Q, et al. Membrane Damage during Ferroptosis Is Caused by Oxidation of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1. Molecular Cell. 2020 2. Li P, Lin Q, Sun S, et al. Inhibition of cannabinoid receptor type 1 sensitizes triple-negative breast cancer cells to ferroptosis via regulating fatty acid metabolism. Cell Death & Disease. 2022, 13(9): 1-15. 3. Bi G, Liang J, Bian Y, et al.Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer.Nature Communications.2024, 15(1): 2461.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Bioactive Compounds Library Max NO PAINS Compound Library Apoptosis Compound Library Ferroptosis Compound Library Anti-COVID-19 Compound Library Cell Cycle Compound Library

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Keywords

FIN56 1083162-61-1 Apoptosis Ferroptosis inhibit GPX4 degradation FIN 56 Inhibitor squalene FIN-56 synthase inhibitor

 

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