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Atglistatin

Catalog No. T1875 CAS 1469924-27-3

Atglistatin is a highly effective, specific and competitive ATGL inhibitor with an IC50 of ~0.7 μM for inhibition of lipolysis in vitro, and no toxicity when the concentration up to 50 μM.

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Atglistatin, CAS 1469924-27-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Atglistatin is a highly effective, specific and competitive ATGL inhibitor with an IC50 of ~0.7 μM for inhibition of lipolysis in vitro, and no toxicity when the concentration up to 50 μM.
Targets&IC50	ATGL:0.7 μM
Kinase Assay	Determination of lipase activity: For determination of lipase activity, lysates are incubated with a substrate containing radiolabeled [9,10-3H(N)]-triolein. Subsequently, FA are extracted and quantitated by liquid scintilation counting. Data are presented as mean S.D. of triplicate determinations and representative for at least three independent experiments.
Cell Research	For MTT-based in vitro viability assays, cells are seeded in 96-well plates and cultured under standard conditions for 24 h. The next day, cells are pretreated with different concentrations of Atglistatin dissolved in DMSO or Cisplatin dissolved in DMF as positive control for 2 h. Medium is replaced by an identical fresh medium and incubated again for the indicated time points. Thereafter, cells are incubated for 3 h with 100 μL Thiazolyl Blue Tetrazolium Bromide (MTT). The resulting violet formazan crystals are dissolved by adding 100 μL of MTT solubilization solution (0.1% NP-40, 4 mM HCl and anhydrous isopropanol). After complete dissolution of the formazan product, absorbance is measured at 595 nm using 690 nm as reference wavelength.(Only for Reference)

Molecular Weight	283.375
Formula	C17H21N3O
CAS No.	1469924-27-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: <1 mg/mL

DMSO: 52 mg/mL (183.5 mM)

Ethanol: 4 mg/mL (14.11 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Mayer N, et al. Nat Chem Biol. 2013, 9(12), 785-787.

Citations

1. Bottermann K, Kalfhues L, Nederlof R, et al. Cardiomyocyte p38 MAPKα suppresses a heart–adipose tissue–neutrophil crosstalk in heart failure development. Basic Research in Cardiology. 2022, 117(1): 1-21. 2. Li M, Yang J, Ye C, et al. Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection. Virologica Sinica. 2021: 1-12.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Metabolism Compound Library Anti-Obesity Compound Library Lipid Metabolism Compound Library Inhibitor Library Anti-Metabolism Disease Compound Library Bioactive Lipid Compound Library Protease Inhibitor Library Target-Focused Phenotypic Screening Library Anti-Aging Compound Library Bioactive Compound Library

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MAGL-IN-4 AA38-3 JZL 184 MJN110 euphol Lalistat 2 NF-1819 JZP-361

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Atglistatin 1469924-27-3 代谢 Lipase ATGL inhibit Adipose triglyceride lipase Inhibitor inhibitor

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