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Anti-PNPLA2 Polyclonal Antibody
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Catalog No. TMAB-01554
Alias TTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase
Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM.
Anti-PNPLA2 Polyclonal Antibody
Anti-PNPLA2 Polyclonal Antibody
😃Good
Catalog No. TMAB-01554Alias TTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase
Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM. |
| Alias | TTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Lane 1: Mouse Brown fat tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-Adipose Triglyceride Lipase (TMAB-01554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 52 kDa 2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (TMAB-01554) Dilution: 3 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 3ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lipid droplet. Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals. |
| Immunogen | KLH conjugated synthetic peptide: human ATGL |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Lipases,Lipases,Metabolic disorders,Obesity,Lipid metabolism |
| Molecular Weight | Theoretical: 55 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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