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Anti-PNPLA2 Polyclonal Antibody

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Catalog No. TMAB-01554
Alias TTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase

Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM.

Anti-PNPLA2 Polyclonal Antibody

Anti-PNPLA2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01554Alias TTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase
Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-PNPLA2 Polyclonal Antibody is a Rabbit antibody targeting PNPLA2. Anti-PNPLA2 Polyclonal Antibody can be used in WB,FCM.
AliasTTS2.2, Transport-secretion protein 2 (TTS2), PNPLA2, Pigment epithelium-derived factor receptor (PEDF-R), Patatin-like phospholipase domain-containing protein 2, Desnutrin, Calcium-independent phospholipase A2-zeta (iPLA2-zeta), ATGL, Adipose triglyceride lipase
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Sample:
Lane 1: Mouse Brown fat tissue lysates
Lane 2: Mouse Heart tissue lysates
Primary: Anti-Adipose Triglyceride Lipase (TMAB-01554) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 52 kDa
2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (TMAB-01554)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 3ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationLipid droplet. Cell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundAdipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ATGL
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Lipases,Lipases,Metabolic disorders,Obesity,Lipid metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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