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7α-Hydroxy-4-cholesten-3-one

Name: 7α-Hydroxy-4-cholesten-3-one
Brand: TargetMol
SKU: T10196
Price: 289 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10196Cas No. 3862-25-7

Alias 7-hydroxy-4-cholesten-3-one

7α-Hydroxy-4-cholesten-3-one is an intermediate in bile acid synthesis from cholesterol, a PXR agonist, and a biomarker for bile acid loss and diseases related to defective bile acid biosynthesis. It is also the physiological substrate for [CYP8B1].

7α-Hydroxy-4-cholesten-3-one

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Purity: 99.14%

Catalog No. T10196Alias 7-hydroxy-4-cholesten-3-oneCas No. 3862-25-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$289	In Stock	In Stock
5 mg	$612	-	In Stock
10 mg	$892	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.14%

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	7α-Hydroxy-4-cholesten-3-one is an intermediate in bile acid synthesis from cholesterol, a PXR agonist, and a biomarker for bile acid loss and diseases related to defective bile acid biosynthesis. It is also the physiological substrate for [CYP8B1].
In vitro	7α-Hydroxy-4-cholesten-3-one is a key intermediate in the bile acid synthesis pathway. Cytochrome P450 7A1 introduces a 7α-hydroxy group to cholesterol, and 3β-hydroxysteroid dehydrogenase then oxidizes and isomerizes the 3-hydroxy group and the Δ5,6-double bond, forming 7α-Hydroxy-4-cholesten-3-one. The absence of P450 27A1 downstream increases its concentration [1].
In vivo	7α-Hydroxy-4-cholesten-3-one significantly influences the hepatic enzyme CYP7A1 activity under steady-state conditions and during rapid diurnal fluctuations in rats. Moreover, its serum levels exhibit a marked diurnal rhythm.
Synonyms	7-hydroxy-4-cholesten-3-one

Chemical Properties

Molecular Weight	400.64
Formula	C₂₇H₄₄O₂
Cas No.	3862-25-7
Smiles	[H][C@@]12CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC[C@@]1([H])[C@@]2([H])[C@H](O)CC2=CC(=O)CC[C@]12C
Relative Density.	1.03 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (199.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (8.24 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4960 mL	12.4800 mL	24.9601 mL	124.8003 mL
5 mM	0.4992 mL	2.4960 mL	4.9920 mL	24.9601 mL
10 mM	0.2496 mL	1.2480 mL	2.4960 mL	12.4800 mL
20 mM	0.1248 mL	0.6240 mL	1.2480 mL	6.2400 mL
50 mM	0.0499 mL	0.2496 mL	0.4992 mL	2.4960 mL
100 mM	0.0250 mL	0.1248 mL	0.2496 mL	1.2480 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc