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7α-Hydroxy-4-cholesten-3-one

Name: 7α-Hydroxy-4-cholesten-3-one
Brand: TargetMol
SKU: T10196
Price: 289 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10196Cas No. 3862-25-7

Alias 7-hydroxy-4-cholesten-3-one

7α-Hydroxy-4-cholesten-3-one is an intermediate in bile acid synthesis from cholesterol, a PXR agonist, and a biomarker for bile acid loss and diseases related to defective bile acid biosynthesis. It is also the physiological substrate for [CYP8B1].

7α-Hydroxy-4-cholesten-3-one

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Purity: 99.14%

Catalog No. T10196Alias 7-hydroxy-4-cholesten-3-oneCas No. 3862-25-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$289	In Stock	In Stock
5 mg	$612	-	In Stock
10 mg	$892	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.14%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	7α-Hydroxy-4-cholesten-3-one is an intermediate in bile acid synthesis from cholesterol, a PXR agonist, and a biomarker for bile acid loss and diseases related to defective bile acid biosynthesis. It is also the physiological substrate for [CYP8B1].
In vitro	7α-Hydroxy-4-cholesten-3-one is a key intermediate in the bile acid synthesis pathway. Cytochrome P450 7A1 introduces a 7α-hydroxy group to cholesterol, and 3β-hydroxysteroid dehydrogenase then oxidizes and isomerizes the 3-hydroxy group and the Δ5,6-double bond, forming 7α-Hydroxy-4-cholesten-3-one. The absence of P450 27A1 downstream increases its concentration [1].
In vivo	7α-Hydroxy-4-cholesten-3-one significantly influences the hepatic enzyme CYP7A1 activity under steady-state conditions and during rapid diurnal fluctuations in rats. Moreover, its serum levels exhibit a marked diurnal rhythm.
Synonyms	7-hydroxy-4-cholesten-3-one

Chemical Properties

Molecular Weight	400.64
Formula	C₂₇H₄₄O₂
Cas No.	3862-25-7
Smiles	[H][C@@]12CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC[C@@]1([H])[C@@]2([H])[C@H](O)CC2=CC(=O)CC[C@]12C
Relative Density.	1.03 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (199.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (8.24 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4960 mL	12.4800 mL	24.9601 mL	124.8003 mL
5 mM	0.4992 mL	2.4960 mL	4.9920 mL	24.9601 mL
10 mM	0.2496 mL	1.2480 mL	2.4960 mL	12.4800 mL
20 mM	0.1248 mL	0.6240 mL	1.2480 mL	6.2400 mL
50 mM	0.0499 mL	0.2496 mL	0.4992 mL	2.4960 mL
100 mM	0.0250 mL	0.1248 mL	0.2496 mL	1.2480 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc