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(+)-Afzelechin

Name: (+)-Afzelechin
Brand: TargetMol
SKU: TN1361
Rating: 4.5 (1 reviews)

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Catalog No. TN1361Cas No. 2545-00-8

Alias Afzelechin

(+)-Afzelechin (Afzelechin) is isolated from Eysenhardtia subcoriacea. It is an α-glucosidase activity inhibitor, offering neuroprotective effects against glutamate-induced neurotoxicity in HT22 cells and improving reduced glutathione levels in rat pancreas homogenates.

(+)-Afzelechin

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Catalog No. TN1361Alias AfzelechinCas No. 2545-00-8

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$458	Inquiry	Inquiry
5 mg	$1,018	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	(+)-Afzelechin (Afzelechin) is isolated from Eysenhardtia subcoriacea. It is an α-glucosidase activity inhibitor, offering neuroprotective effects against glutamate-induced neurotoxicity in HT22 cells and improving reduced glutathione levels in rat pancreas homogenates.
Targets&IC50	α-glucosidase:0.13 mM(ID50)
In vitro	Treatment of human umbilical vein endothelial cells (HUVECs) with (+)-Afzelechin (20 μM, 6h) effectively inhibited the production of iNOS and prevented LPS-induced NO synthesis. (+)-AfzelechinC (2-20 μM, 6h) demonstrated its anti-inflammatory ability by down-regulating IL-1β expression in LPS-treated HUVEC. [1] (+)-Afzelechin improves the efficacy of sepsis induced ALI by modulating TLR4/NF-κB, PI3K/Akt, Hippo, and Rho signaling pathways to reduce inflammation. [2]
In vivo	(+)-Afzelechin (0.04-0.4 mg/kg, intravenously), results showed significantly reduced iNOS expression in mice with LPS-induced lung injury models, indicating the anti-inflammatory activity of (+)-Afzelechin in vivo. [1]
Synonyms	Afzelechin

Chemical Properties

Molecular Weight	274.27
Formula	C₁₅H₁₄O₅
Cas No.	2545-00-8
Smiles	OC1=C2C(O[C@@H]([C@@H](O)C2)C3=CC=C(O)C=C3)=CC(O)=C1
Relative Density.	1.492 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (291.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (12.03 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6460 mL	18.2302 mL	36.4604 mL	182.3021 mL
5 mM	0.7292 mL	3.6460 mL	7.2921 mL	36.4604 mL
10 mM	0.3646 mL	1.8230 mL	3.6460 mL	18.2302 mL
20 mM	0.1823 mL	0.9115 mL	1.8230 mL	9.1151 mL
50 mM	0.0729 mL	0.3646 mL	0.7292 mL	3.6460 mL
100 mM	0.0365 mL	0.1823 mL	0.3646 mL	1.8230 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc