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TNF alpha Protein, Mouse, Recombinant
(Synonyms: tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF) Copy Product InfoSynonyms: tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF
Catalog No. TMPY-01294 Copy Product Info
Purity: 97.5%
TNF alpha Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.39 kDa and the accession number is P06804.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $54 | - | In Stock | |
| 10 μg | $84 | - | In Stock | |
| 20 μg | $136 | In Stock | In Stock | |
| 50 μg | $228 | 7-10 days | 7-10 days | |
| 100 μg | $346 | 7-10 days | 7-10 days | |
| 200 μg | $613 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:97.5%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.  |
| Description | TNF alpha Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.39 kDa and the accession number is P06804. |
| Species | Mouse |
| Expression System | E. coli |
| Tag | Tag Free |
| Accession Number | P06804 |
| Construction | A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the N-terminus. Predicted N terminal: Met |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF |
| Research Background |
| Molecular Weight | 17.39 kDa (predicted); 17.39 kDa (reducing conditions) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: TNF alpha Protein, Mouse, Recombinant chemical structure | TNF alpha Protein, Mouse, Recombinant molecular weight
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