TNF alpha Protein, Rat, Recombinant

Name: TNF alpha Protein, Rat, Recombinant
Brand: TargetMol
SKU: TMPY-02202
Price: 54 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: tumor necrosis factor, TNF-α) Copy Product Info

Synonyms: tumor necrosis factor, TNF-α

Catalog No. TMPY-02202 Copy Product Info

Purity: 98.2%

TNF alpha Protein, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.4 kDa and the accession number is P16599.

Pack Size	Price	USA Stock	Global Stock
5 µg	$54	-	In Stock
10 µg	$85	-	In Stock
20 µg	$137	In Stock	In Stock
50 µg	$275	7-10 days	7-10 days
100 µg	$472	7-10 days	7-10 days
200 µg	$803	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.2%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-6 pg/ml.
Description	TNF alpha Protein, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.4 kDa and the accession number is P16599.
Species	Rat
Expression System	E. coli
Tag	Tag Free
Accession Number	P16599
Construction	A DNA sequence encoding the mature form of rat TNFα (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus. Predicted N terminal: Met
Protein Purity	> 93 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	tumor necrosis factor, TNF-α
Research Background	Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

17.4 kDa (predicted); 15.9 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.