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Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 2, Tumor Necrosis Factor, TNF-α, TNFSF2, TNF-Alpha, TNF-a, TNFA, TNF α, TNF, Cachectin

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $35 | - | In Stock | |
| 10 μg | $53 | - | In Stock | |
| 20 μg | $77 | - | In Stock | |
| 50 μg | $129 | - | In Stock | |
| 100 μg | $215 | - | In Stock | |
| 200 μg | $363 | 7-10 days | 7-10 days | |
| 500 μg | $739 | 7-10 days | 7-10 days | |
| 1 mg | $1,060 | 7-10 days | 7-10 days |
| Bioactivity | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-40 pg/ml. (QC verified) ![]() |
| Description | TNF alpha Protein, Human, Recombinant (aa 77-233, His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16 KDa and the accession number is P01375. |
| Species | Human |
| Expression System | E. coli |
| Tag | C-6xHis |
| Accession Number | P01375 |
| Amino Acid | Val77-Leu233 |
| Construction | Val77-Leu233 |
| Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) ![]() |
| Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 150 mM NaCl, pH 8.0. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Tumor Necrosis Factor Ligand Superfamily Member 2, Tumor Necrosis Factor, TNF-α, TNFSF2, TNF-Alpha, TNF-a, TNFA, TNF α, TNF, Cachectin |
| Research Background |
| Molecular Weight | 16 KDa (reducing condition) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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