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TNF alpha Protein, Human, Recombinant (aa 77-233, His)

(Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 2, Tumor Necrosis Factor, TNF-α, TNFSF2, TNF-Alpha, TNF-a, TNFA, TNF α, TNF, Cachectin) Copy Product Info

Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 2, Tumor Necrosis Factor, TNF-α, TNFSF2, TNF-Alpha, TNF-a, TNFA, TNF α, TNF, Cachectin

Catalog No. TMPJ-00124 Copy Product Info
TNF alpha Protein, Human, Recombinant (aa 77-233, His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16 KDa and the accession number is P01375.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$35-In Stock
10 μg$53-In Stock
20 μg$77-In Stock
50 μg$129-In Stock
100 μg$215-In Stock
200 μg$3637-10 days7-10 days
500 μg$7397-10 days7-10 days
1 mg$1,0607-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:Greater than 95% as determined by reducing SDS-PAGE.
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-40 pg/ml. (QC verified)
TNF alpha Protein, Human, Recombinant (aa 77-233, His)
Description
TNF alpha Protein, Human, Recombinant (aa 77-233, His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16 KDa and the accession number is P01375.
Species
Human
Expression System
E. coli
TagC-6xHis
Accession NumberP01375
Amino AcidVal77-Leu233
ConstructionVal77-Leu233
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
TNF alpha Protein, Human, Recombinant (aa 77-233, His)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTumor Necrosis Factor Ligand Superfamily Member 2, Tumor Necrosis Factor, TNF-α, TNFSF2, TNF-Alpha, TNF-a, TNFA, TNF α, TNF, Cachectin
Research Background
Chemical Properties
Molecular Weight16 KDa (reducing condition)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: TNF alpha Protein, Human, Recombinant (aa 77-233, His) chemical structure | TNF alpha Protein, Human, Recombinant (aa 77-233, His) molecular weight