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CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (hFc & His & Flag)

(Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product Info

Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

Catalog No. TMPY-06108 Copy Product Info
CD3D & CD3E Heterodimer Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 75.4 kDa and the accession number is P04235&P22646.

Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD3D & CD3E Heterodimer Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 75.4 kDa and the accession number is P04235&P22646.
Species
Mouse
Expression System
HEK293 Cells
TagC-hFc & His & C-hFc & Flag
Accession NumberP04235&P22646
ConstructionA DNA sequence encoding the extracellular domain (Met1-Ala105) of mouse CD3D (P04235) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp108) of mouse CD3E (P22646) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Asp 23
Protein Purity
> 90 % as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsT3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Chemical Properties
Molecular Weight75.4 kDa (predicted); 36-55 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (hFc & His & Flag) chemical structure | CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (hFc & His & Flag) molecular weight