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CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 78.4 kDa and the accession number is NP_000724.1&NP_000064.1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $583 | In Stock | |
200 μg | $1,030 | 7-10 days | |
500 μg | $2,170 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 78.4 kDa and the accession number is NP_000724.1&NP_000064.1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-Fc, C-Fc |
Accession Number | NP_000724.1&NP_000064.1 |
Synonyms | TCRE,T3E,IMD18,CD3e molecule, ε (CD3-TCR complex),CD3e molecule, epsilon (CD3-TCR complex),CD3E & CD3G Heterodimer |
Construction | A DNA sequence encoding the human CD3E (NP_000724.1) (Met1-Asp126) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human CD3G (NP_000064.1) (Met1-Ser113) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. Predicted N terminal: Asp 23 & Gln 23 |
Protein Purity | > 90 % as determined by SDS-PAGE. ![]() |
Molecular Weight | 78.4 kDa (predicted) |
Endotoxin | < 0.01 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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