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CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc)

(Synonyms: TCRE, T3E, IMD18, CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3E & CD3G Heterodimer) Copy Product Info

Synonyms: TCRE, T3E, IMD18, CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3E & CD3G Heterodimer

Catalog No. TMPY-05457 Copy Product Info
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 78.4 kDa and the accession number is NP_000724.1&NP_000064.1.

Batch Information

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Purity:93.5%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 78.4 kDa and the accession number is NP_000724.1&NP_000064.1.
Species
Human
Expression System
HEK293 Cells
TagC-Fc, C-Fc
Accession NumberNP_000724.1&NP_000064.1
ConstructionA DNA sequence encoding the human CD3E (NP_000724.1) (Met1-Asp126) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human CD3G (NP_000064.1) (Met1-Ser113) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. Predicted N terminal: Asp 23 & Gln 23
Protein Purity
> 90 % as determined by SDS-PAGE.
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc)
Endotoxin< 0.01 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsTCRE, T3E, IMD18, CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3E & CD3G Heterodimer
Chemical Properties
Molecular Weight78.4 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc) chemical structure | CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc) molecular weight