CD3 epsilon/CD3e Protein, Rat, Recombinant (hFc)

Name: CD3 epsilon/CD3e Protein, Rat, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-00628
Rating: 4.5 (1 reviews)

(Synonyms: CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e) Copy Product Info

Synonyms: CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e

Catalog No. TMPY-00628 Copy Product Info

CD3 epsilon/CD3e Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 36.1 kDa and the accession number is A6J423.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD3 epsilon/CD3e Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 36.1 kDa and the accession number is A6J423.
Species	Rat
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	A6J423
Construction	A DNA sequence encoding the rat Cd3e (NP_001101610.1) (Tyr24-Asp103) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Tyr 24
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e
Research Background	T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

36.1 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.