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CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc)

Catalog No. TMPY-04010
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc) is expressed in HEK293 mammalian cells with Fc-Flag, mFc tag. The predicted molecular weight is 76.9 kDa and the accession number is NP_000724.1&NP_000064.1.
Pack SizePriceAvailabilityQuantity
50 μg $313In Stock
100 μg $5537-10 days
200 μg $9777-10 days
500 μg $2,0607-10 days
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc) is expressed in HEK293 mammalian cells with Fc-Flag, mFc tag. The predicted molecular weight is 76.9 kDa and the accession number is NP_000724.1&NP_000064.1.
Species
Human
Expression System
HEK293 Cells
TagC-hFc-Flag, C-mFc
Accession NumberNP_000724.1&NP_000064.1
Synonyms
TCRE,T3E,IMD18,CD3e molecule, ε (CD3-TCR complex),CD3e molecule, epsilon (CD3-TCR complex),CD3E & CD3G Heterodimer
Construction
A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal FLAG-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. Predicted N terminal: Asp 23 & Gln 23
Protein Purity
> 95 % as determined by SDS-PAGE
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc)
Molecular Weight76.9 kDa (predicted); 42-47 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.

Dose Conversion

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