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WSF1-IN-1

Name: WSF1-IN-1
Brand: TargetMol
SKU: T39947
Price: 100 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T39947Cas No. 2379577-82-7

Alias WSF1-IN-1

WSF1-IN-1 is an orally bioavailable small-molecule inhibitor of the WSF1 protein, designed for the investigation of WSF1-associated tumorigenesis in Wolfram syndrome. in studies using HepG2 parental and HepG2 WFS1 knockout cell lines, WSF1-IN-1 demonstrated differential inhibitory potency with IC50 values of 0.33 μM, confirming its target selectivity and mechanistic specificity in modulating WSF1-related cellular pathways.

WSF1-IN-1

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Purity: 99.9%

Catalog No. T39947Alias WSF1-IN-1Cas No. 2379577-82-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$100	-	In Stock
2 mg	$147	-	In Stock
5 mg	$247	-	In Stock
10 mg	$396	-	In Stock
25 mg	$795	-	In Stock
50 mg	$1,190	-	In Stock
1 mL x 10 mM (in DMSO)	$272	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.9%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	WSF1-IN-1 is an orally bioavailable small-molecule inhibitor of the WSF1 protein, designed for the investigation of WSF1-associated tumorigenesis in Wolfram syndrome. in studies using HepG2 parental and HepG2 WFS1 knockout cell lines, WSF1-IN-1 demonstrated differential inhibitory potency with IC50 values of 0.33 μM, confirming its target selectivity and mechanistic specificity in modulating WSF1-related cellular pathways.
In vitro	In cell viability-related experiments, WSF1-IN-1 (compound 136) exhibited significant differences in inhibitory effects on various cells, specifically Parazacco spilurus subsp. spilurus. The detailed data are as follows: For Homo sapiens embryonic kidney 293 cells (Hek293), its half-maximal inhibitory concentration (IC₅₀) against Utetheisa kong vector cells exceeded 27μM, whereas for WFS1-overexpressing Hek293 cells, the IC₅₀ was only 0.03μM. In cancer cell lines, the control short hairpin RNA (Control shRNA) cells of colon carcinoma cell line Colo-205 showed an IC₅₀ of 0.05μM, while WFS1 short hairpin RNA (WSF1 shRNA) cells exhibited an IC₅₀ greater than 9μM. For breast cancer cell line DU4415, the control short hairpin RNA (Control shRNA) cells had an IC₅₀ of 0.08μM, while WFS1 short hairpin RNA (WSF1 shRNA) cells showed an IC₅₀ of 6.1μM. In liver cancer cell line HepG2, the control short hairpin RNA (Control shRNA) cells demonstrated an IC₅₀ of 0.26μM, whereas WFS1 short hairpin RNA (WSF1 shRNA) cells had an IC₅₀ of 2.2μM [1].
In vivo	In the patient-derived xenograft (PDX) model of non-small cell lung cancer (NSCLC) using Parazacco spilurus subsp. Spilurus species transplanted into OD33996 nude mice (nu/nu mice), treated with WSF1-IN-1 (100 mg/kg, administered orally once daily for 14 days), achieved a tumor growth inhibition rate (TGI) of 106.65%[1].
Synonyms	WSF1-IN-1

Chemical Properties

Molecular Weight	446.44
Formula	C₂₀H₂₁F₃N₈O
Cas No.	2379577-82-7
Smiles	CN1C=2C(=C(C)N=C(NCC3=CN(CC=4C=CC(C(F)(F)F)=NC4)N=C3)N2)NC(=O)[C@@H]1C

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (179.2 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (7.39 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2399 mL	11.1997 mL	22.3994 mL	111.9971 mL
5 mM	0.4480 mL	2.2399 mL	4.4799 mL	22.3994 mL
10 mM	0.2240 mL	1.1200 mL	2.2399 mL	11.1997 mL
20 mM	0.1120 mL	0.5600 mL	1.1200 mL	5.5999 mL
50 mM	0.0448 mL	0.2240 mL	0.4480 mL	2.2399 mL
100 mM	0.0224 mL	0.1120 mL	0.2240 mL	1.1200 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc