Verbascose is an oligosaccharide and non-reducing sugar that can regulate and enhance the immune function of RAW264.7 macrophages, increasing their proliferation activity, phagocytic activity, NO release, and cytokine secretion levels, and also has the potential to regulate intestinal microecological balance.

Method:
RAW264.7 cells were treated with different concentrations of Verbascose (25, 50, 100, 200, and 400 μg/mL) for 12, 24, 36, and 48 hours, and cell viability was assessed.
Result:
Verbascose significantly increased the viability of RAW264.7 cells in the range of 25–400 μg/mL in a time-dependent manner [1].

Methods:
Immunosuppressed female Kunming mice were subcutaneously injected with different doses of Verbascose (30, 90, 120 mg/kg) once daily for 8 consecutive days.
Results:
Treatment with the medium dose (90 mg/kg) significantly increased the spleen index, lysozyme activity in the spleen and serum, serum hemolysin levels, and the earlap swelling rate in the delayed-type hypersensitivity (DTH) response.