This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
Tubulin polymerization-IN-36
Catalog No. T60758 CAS
2011784-91-9
Tubulin polymerization-IN-36 is able to be used in cancer research, for example, lymphomas. Tubulin polymerization-IN-36 binds to the colchicine site of tubulin and inhibits colchicine binding, that is an inhibitor of tubulin polymerization with IC50 of 2.8 μΜ[1].
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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
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Biological Description
Chemical Properties
Storage
& Solubility Information
Description
Tubulin polymerization-IN-36 is able to be used in cancer research, for example, lymphomas. Tubulin polymerization-IN-36 binds to the colchicine site of tubulin and inhibits colchicine binding, that is an inhibitor of tubulin polymerization with IC50 of 2.8 μΜ[1].
In vitro
Tubulin polymerization-IN-36 (compound 2e) demonstrates significant biological activity by inhibiting lymphoma cell proliferation below 50% at a concentration of 1 μM over 72 hours and displaying cytotoxic effects against MCF-7 cells with an IC50 value of 0.29 μM. At concentrations between 50 and 500 nM over periods of 24 to 72 hours, it induces apoptosis and arrests VL51 and MINO cells in the G2/M phase of the cell cycle. Additionally, at 5 μM, it inhibits colchicine’s ability to bind to tubulin by 88%. Cell proliferation assays on VL51, MINO, HBL1, and SU-DHL-10 cell lines with concentrations up to 10 μM for 72 hours show inhibited cell proliferation to 38%, 0.8%, 7.1%, and 30.5% at 1 μM, respectively, with IC50 values ranging from 0.02 to 0.04 μM. Cell cycle analysis further confirms its mechanism of action by arresting VL51 and MINO cells in the G2/M phase at 50 and 500 nM.
Molecular Weight
310.35
Formula
C18H18N2O3
CAS No.
2011784-91-9
Storage
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dose Conversion
You can also refer to dose conversion for different animals.
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Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.