Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SK1-I hydrochloride (BML-258 hydrochloride) is a sphingosine analog that can enhance autophagy and has antitumor activity. SK1-I hydrochloride is an isozyme-specific competitive SPHK1 inhibitor (Ki = 10 μM) [1] [2].
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | 6-8 weeks | $ 332.00 | |
25 mg | 6-8 weeks | $ 1,080.00 | |
50 mg | 6-8 weeks | $ 1,410.00 | |
100 mg | 6-8 weeks | $ 1,990.00 |
Description | SK1-I hydrochloride (BML-258 hydrochloride) is a sphingosine analog that can enhance autophagy and has antitumor activity. SK1-I hydrochloride is an isozyme-specific competitive SPHK1 inhibitor (Ki = 10 μM) [1] [2]. |
In vitro | SK1-I hydrochloride (0-10 μM; 24 hours) attenuates cancer cell growth and survival in a TP53-dependent manner in HCT116 cells and HCT116 cells bearing TP53 null cancer [2]. SK1-I hydrochloride (0-20 μM; 12 hours) induces more CASP3 cleavage in HCT116 cells, compared to HCT116 cells lacking TP53, leading to a hallmark of apoptosis [2]. Cell Viability Assay [2] Cell Line: HCT116 cells and HCT116 cells bearing TP53 null cancer Concentration: 0 μM, 2.5 μM, 5 μM, 7.5 μM, 10 μM Incubation Time: 24 hours Result: Decreased cancer cell growth and survival. Western Blot Analysis [2] Cell Line: HCT116 cells and HCT116 cells bearing TP53 null cancer Concentration: 0 μM, 5 μM, 10 μM, 20 μM Incubation Time: 12 hours Result: Induced more CASP3 cleavage in HCT116 cells, compared to HCT116 cells lacking TP53. |
In vivo | Pre-treatment with SK1-I hydrochloride (also known as BML-258 hydrochloride) through intraperitoneal (i.p.) injection at a dosage of 75 mg/kg, administered once 24 hours before baseline mean arterial blood pressure (MAP) measurement, significantly reduces the hypotensive effect induced by subsequent anandamide administration (delivered via i.v. injection in two doses of 1 and 10 mg/kg). This observation was made in male C57BL/6 mice weighing 24 ± 3.5 g. This regimen not only highlights the drug's potential impact on blood pressure modulation but also demonstrates a marked decrease in baseline MAP, indicating a notable interaction between SK1-I hydrochloride and anandamide in affecting vascular responses. |
Molecular Weight | 313.86 |
Formula | C17H28ClNO2 |
CAS No. | 2366222-05-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
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SK1- I hydrochloride 2366222-05-9 inhibitor inhibit